Follow SiliCycle on Facebook Follow SiliCycle on Twitter Follow SiliCycle on LinkedIn Watch our videos on Youtube

Aminoglycoside Antibiotics

> back to Suggested SPE Protocols


Milk, meat (beef, chicken, pork) and eggs


Spectinomycin, Apramycin, Dihydrostreptomycin, Gentamicin, Hygromycin B, Kanamycin, Neomycin B, Streptomycin, Amikacin, Netilmicin, Paromomycin, Sisomycin, Tobramycin

SPE Cartridge

SiliaPrepX WCX
500 mg / 6 mL

Sample Pretreatment before Loading

Weigh 5 g of mixed eggs or frozen and milled meat samples, or 10 mL of bovine milk.
Add 20 mL of the extraction solution: NH4OAc (10 mM), EDTA (0.4 mM), NaCl (0.5 %) and Trichloroacetic Acid (2 %) in Water.
Vortex and centrifugate at 4,000 rpm for 10 minutes.
Transfer the supernatant to a clean tube.
Repeat extraction and combine supernatants.
Adjust pH to 6.5.


10 mL of Methanol


10 mL of Water


Pretreated sample


10 mL of Ammonium Acetate buffer (50 mM, pH 7.0)
Dry the cartridge


6 mL of 2 % Formic Acid in Water

Further Treatment

Dilute with 4 mL of Water, filter on 0.2 μm and quantification by LC/MS/MS.

1 Item(s)

Set Descending Direction
per page

List  Grid 

  1. SiliaPrepX WCX Polymeric SPE Cartridges, 85 µm, 60 Å (SPE-P0015)

    SiliaPrepX Polymeric SPE Cartridges, Carboxylic Acid (WCX), 85 µm, 60 Å (SPE-P0015)

    SiliaPrepX WCX is a polystyrene-divinylbenzene copolymer functionalized by a weak cation exchanger used to catch and release strong basic compounds (pKa >10). It is highly stable in organic solvents.

1 Item(s)

Set Descending Direction
per page

List  Grid