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Barbiturates

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Matrix

Biological fluids (blood, plasma, serum, urine or tissue)

Analytes

Phenobarbital, Butalbital, Amobarbital, Pentobarbital, Secobarbital, Butabarbital, Hexobarbital

SPE Cartridge

SiliaPrep CleanDRUG
200 mg / 6 mL
SPEC-R651230B-06G

Sample Pretreatment before Loading

Add 3 mL of Phosphate buffer (100 mM, pH 6.0) to 2 mL of blood / plasma / serum / urine (or 1 g of tissue homogenate).
Mix / vortex.
Adjust pH to 6.0 ± 0.5 with Sodium Phosphate (100 mM).
Centrifugate at 2,000 rpm for 10 minutes and discard cellular debris.

Conditionning

3 mL of Methanol

Equilibration

3 mL of Phosphate buffer (100 mM, pH 6.0)

Loading

Pretreated sample

Washing

3 mL of Water
1 mL of Acetic Acid (100 mM) Dry the cartridge
2 mL of Hexane

Elution

3 mL of Ethyl Acetate / Hexane (50:50)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.
Or dissolution in Ethyl Acetate and quantification by GC/MS.

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  1. SiliaPrep SPE Cartridges CleanDRUG (SPEC-R651230B)

    SiliaPrep SPE Cartridges, CleanDRUG (SPEC-R651230B)

    SiliaPrep CleanDRUG phase has been optimized for drugs of abuse applications

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