Benzodiazepines

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Matrix

Urine

Analytes

Nordiazepam, 7-Aminoclonazepam, Desalkylflurazepam, Temazepam, Alprazolam, Clonazepam, Midazolam, Flurazepam, 7-Aminoflunitrazepam, Diazepam, Oxazepam, Chlordiazepoxide, Flunitrazepam, Lorazepam

SPE Cartridge

SiliaPrepX SCX
30 mg / 3 mL
SPE-P0005-03AA

Sample Pretreatment before Loading

Dilute 1 mL of urine with 2 mL of 2 % Formic Acid.

Conditionning

1 mL of Methanol

Equilibration

1 mL of Water

Loading

Pretreated sample

Washing

2 mL of 2 % Formic Acid
2 mL of Methanol / Water (50:50)

Elution

1 mL of 5 % Ammonium Hydroxide in Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Methanol and quantification by LC/MS/MS.