Benzodiazepines
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Matrix
Urine
Analytes
Nordiazepam, 7-Aminoclonazepam, Desalkylflurazepam, Temazepam, Alprazolam, Clonazepam, Midazolam, Flurazepam, 7-Aminoflunitrazepam, Diazepam, Oxazepam, Chlordiazepoxide, Flunitrazepam, Lorazepam
SPE Cartridge
SiliaPrepX SCX
30 mg / 3 mL
SPE-P0005-03AA
Sample Pretreatment before Loading
Dilute 1 mL of urine with 2 mL of 2 % Formic Acid.
Conditionning
1 mL of Methanol
Equilibration
1 mL of Water
Loading
Pretreated sample
Washing
2 mL of 2 % Formic Acid
2 mL of Methanol / Water (50:50)
Elution
1 mL of 5 % Ammonium Hydroxide in Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Methanol and quantification by LC/MS/MS.
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SiliaPrepX Polymeric SPE Cartridges, Tosic Acid (SCX), 85 µm, 60 Å (SPE-P0005)
SiliaPrepX SCX is a polystyrene-divinylbenzene copolymer functionalized by a strong cation exchanger presenting a high selectivity for acids (pKa 2 - 10). It is highly stable in organic solvents.