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Biomarkers of Alcohol Consumption
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Matrix
Urine
Analytes
Ethyl Glucuronide (EtG), Ethyl Sulfate (EtS)
SPE Cartridge
SiliaPrep Amine (WAX)
100 mg / 1 mL
SPE-R52030B-01C
Sample Pretreatment before Loading
Add 50 μL of HCl (6 M) and 1 mL of Acetonitrile to 100 μL of urine, centrifugate.
Conditionning
2 mL of Methanol
Equilibration
2 mL of Water
2 mL of 0.2 % Acetic Acid in Acetonitrile
Loading
Pretreated sample
Washing
1 mL of Hexane
Dry the cartridge
Elution
2 x 750 μL of 2 % Ammonium Hydroxide in Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with Water and quantification by LC/MS/MS.
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SiliaPrep SPE Cartridges Amine (WAX) (SPE-R52030B)
SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.
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