Biomarkers of Alcohol Consumption

> back to Suggested SPE Protocols

Matrix

Urine

Analytes

Ethyl Glucuronide (EtG), Ethyl Sulfate (EtS)

SPE Cartridge

SiliaPrep Amine (WAX)
100 mg / 1 mL
SPE-R52030B-01C

Sample Pretreatment before Loading

Add 50 μL of HCl (6 M) and 1 mL of Acetonitrile to 100 μL of urine, centrifugate.

Conditionning

2 mL of Methanol

Equilibration

2 mL of Water
2 mL of 0.2 % Acetic Acid in Acetonitrile

Loading

Pretreated sample

Washing

1 mL of Hexane
Dry the cartridge

Elution

2 x 750 μL of 2 % Ammonium Hydroxide in Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with Water and quantification by LC/MS/MS.