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Biomarkers of Alcohol Consumption

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Matrix

Urine

Analytes

Ethyl Glucuronide (EtG), Ethyl Sulfate (EtS)

SPE Cartridge

SiliaPrep Amine (WAX)
100 mg / 1 mL
SPE-R52030B-01C

Sample Pretreatment before Loading

Add 50 μL of HCl (6 M) and 1 mL of Acetonitrile to 100 μL of urine, centrifugate.

Conditionning

2 mL of Methanol

Equilibration

2 mL of Water
2 mL of 0.2 % Acetic Acid in Acetonitrile

Loading

Pretreated sample

Washing

1 mL of Hexane
Dry the cartridge

Elution

2 x 750 μL of 2 % Ammonium Hydroxide in Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with Water and quantification by LC/MS/MS.

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  1. SiliaPrep SPE Cartridges Amine (WAX) (SPE-R52030B)

    SiliaPrep SPE Cartridges Amine (WAX) (SPE-R52030B)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.

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