Buprenorphine & Norbuprenorphine
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Matrix
Whole blood
Analytes
Buprenorphine, Norbuprenorphine
SPE Cartridge
SiliaPrepX SCX
30 mg / 3 mL
SPE-P0005-03AA
Sample Pretreatment before Loading
Dilute 0.5 mL of blood with 3 mL of Phosphate buffer (0.1 M, pH 6.0). Vortex, centrifugate and discard cellular debris.
Conditionning
3 mL of Methanol
Equilibration
3 mL of Water
Loading
Pretreated sample
Washing
2 x 2 mL of 2 % Formic Acid
3 mL of Methanol / 2 % Formic Acid (70:30)
Dry the cartridge
Elution
2 x 1.5 mL of 5 % Ammonium Hydroxide in Ethyl Acetate / Isopropanol (80:20)
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Methanol / Water (15:85) and quantification by LC/MS/MS.
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SiliaPrepX Polymeric SPE Cartridges, Tosic Acid (SCX), 85 µm, 60 Å (SPE-P0005)
SiliaPrepX SCX is a polystyrene-divinylbenzene copolymer functionalized by a strong cation exchanger presenting a high selectivity for acids (pKa 2 - 10). It is highly stable in organic solvents.