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Caffeine, Theophylline & Theobromine

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Biological fluids (blood, plasma, serum, urine or tissue)


Caffeine, Theophylline, Theobromine

SPE Cartridge

SiliaPrep CleanDRUG
200 mg / 6 mL

Sample Pretreatment before Loading

Add 3 mL of Acetic Acid (100 mM) to 1 mL of blood / serum / plasma / urine.
Vortex, centrifugate.


3 mL of Methanol
3 mL of Water


1 mL of Acetic Acid (100 mM)


Pretreated sample


3 mL of Water
3 mL of Acetic Acid (100 mM)
Dry the cartridge


3 mL of Ethyl Acetate / Methanol (90:10)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and identification by HPLC.

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  1. SiliaPrep SPE Cartridges CleanDRUG (SPEC-R651230B)

    SiliaPrep SPE Cartridges, CleanDRUG (SPEC-R651230B)

    SiliaPrep CleanDRUG phase has been optimized for drugs of abuse applications

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