Caffeine, Theophylline & Theobromine

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Matrix

Biological fluids (blood, plasma, serum, urine or tissue)

Analytes

Caffeine, Theophylline, Theobromine

SPE Cartridge

SiliaPrep CleanDRUG
200 mg / 6 mL
SPEC-R651230B-06G

Sample Pretreatment before Loading

Add 3 mL of Acetic Acid (100 mM) to 1 mL of blood / serum / plasma / urine.
Vortex, centrifugate.

Conditionning

3 mL of Methanol
3 mL of Water

Equilibration

1 mL of Acetic Acid (100 mM)

Loading

Pretreated sample

Washing

3 mL of Water
3 mL of Acetic Acid (100 mM)
Dry the cartridge

Elution

3 mL of Ethyl Acetate / Methanol (90:10)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and identification by HPLC.