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Caffeine, Theophylline & Theobromine
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Matrix
Biological fluids (blood, plasma, serum, urine or tissue)
Analytes
Caffeine, Theophylline, Theobromine
SPE Cartridge
SiliaPrep CleanDRUG
200 mg / 6 mL
SPEC-R651230B-06G
Sample Pretreatment before Loading
Add 3 mL of Acetic Acid (100 mM) to 1 mL of blood / serum / plasma / urine.
Vortex, centrifugate.
Conditionning
3 mL of Methanol
3 mL of Water
Equilibration
1 mL of Acetic Acid (100 mM)
Loading
Pretreated sample
Washing
3 mL of Water
3 mL of Acetic Acid (100 mM)
Dry the cartridge
Elution
3 mL of Ethyl Acetate / Methanol (90:10)
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and identification by HPLC.
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SiliaPrep SPE Cartridges, CleanDRUG (SPEC-R651230B)
SiliaPrep CleanDRUG phase has been optimized for drugs of abuse applications