Cocaine and Metabolites

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Matrix

Biological fluids (whole blood, urine or plasma)

Analytes

Ecgonine Methyl Ester, Anhydroecgonine Methyl Ester, Benzoylecgonine, Norcocaine, Cocaine, Cocaethylene

SPE Cartridge

SiliaPrepX SCX
1 mL / 30 mg
SPE-P0005-01AA

Sample Pretreatment before Loading

Plasma and Urine: dilute 100 μL of sample with 300 μL of Ammonium Acetate buffer (50 mM, pH 6.0).

Whole blood: lyse red blood cells by 10 minutes sonication in buffer, centrifugate at 11,000 rpm for 10 minutes, discard cellular debris.

Conditionning

1 mL of Methanol

Equilibration

1 mL of Ammonium Acetate (50 mM, pH 6.0)

Loading

Pretreated sample

Washing

1 mL of Ammonium Acetate buffer (50 mM, pH 6.0)
1 mL of 2 % Formic Acid in Water
1 mL of Methanol

Elution

2 x 0.5 mL of 5 % Ammonium Hydroxide in Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with Water / Methanol (80:20) and quantification by LC/MS.