Cocaine and Metabolites
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Matrix
Biological fluids (whole blood, urine or plasma)
Analytes
Ecgonine Methyl Ester, Anhydroecgonine Methyl Ester, Benzoylecgonine, Norcocaine, Cocaine, Cocaethylene
SPE Cartridge
SiliaPrepX SCX
1 mL / 30 mg
SPE-P0005-01AA
Sample Pretreatment before Loading
Plasma and Urine: dilute 100 μL of sample with 300 μL of Ammonium Acetate buffer (50 mM, pH 6.0).
Whole blood: lyse red blood cells by 10 minutes sonication in buffer, centrifugate at 11,000 rpm for 10 minutes, discard cellular debris.
Conditionning
1 mL of Methanol
Equilibration
1 mL of Ammonium Acetate (50 mM, pH 6.0)
Loading
Pretreated sample
Washing
1 mL of Ammonium Acetate buffer (50 mM, pH 6.0)
1 mL of 2 % Formic Acid in Water
1 mL of Methanol
Elution
2 x 0.5 mL of 5 % Ammonium Hydroxide in Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with Water / Methanol (80:20) and quantification by LC/MS.
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SiliaPrepX Polymeric SPE Cartridges, Tosic Acid (SCX), 85 µm, 60 Å (SPE-P0005)
SiliaPrepX SCX is a polystyrene-divinylbenzene copolymer functionalized by a strong cation exchanger presenting a high selectivity for acids (pKa 2 - 10). It is highly stable in organic solvents.