Fatty acid methyl esters (FAMEs)
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Matrix
Olive oil
Analytes
C11:0, C16:0, C16:1 cis 9, C18:0, C18:1 cis 9, C18:2 cis 9,12, C18:3 cis 9,12,15, C20:0, C20:1 cis 11
SPE Cartridge
SiliaPrep Silica
1 g / 6 mL
SPE-R10030B-06S
Sample Pretreatment before Loading
Dilute 0.12 g of oil in 0.5 mL of Hexane.
Conditionning
3 mL of Methanol
3 mL of Water
Equilibration
6 mL of Hexane
Loading
Pretreated sample
Washing
No washing required
Elution
10 mL of Hexane / Diethyl Ether (87:13)
Further Treatment
Evaporation under Nitrogen, reconstitution with Hexane, esterification and identification by GC/FID.
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SiliaPrep SPE Cartridges, Silica, 40 - 63 µm, 60 Å (SPE-R10030B)
SiliaPrep Silica is the most polar sorbent presenting a slightly acidic character used to extract various compounds from non-polar solvents using hydrogen bonding. Efficient sorbent for the separation of analytes with similar structures and for removing the baseline noise from organic samples.