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Fatty acid methyl esters (FAMEs)

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Matrix

Olive oil

Analytes

C11:0, C16:0, C16:1 cis 9, C18:0, C18:1 cis 9, C18:2 cis 9,12, C18:3 cis 9,12,15, C20:0, C20:1 cis 11

SPE Cartridge

SiliaPrep Silica
1 g / 6 mL
SPE-R10030B-06S

Sample Pretreatment before Loading

Dilute 0.12 g of oil in 0.5 mL of Hexane.

Conditionning

3 mL of Methanol
3 mL of Water

Equilibration

6 mL of Hexane

Loading

Pretreated sample

Washing

No washing required

Elution

10 mL of Hexane / Diethyl Ether (87:13)

Further Treatment

Evaporation under Nitrogen, reconstitution with Hexane, esterification and identification by GC/FID.

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  1. SiliaPrep SPE Cartridges Silica (SPE-R10030B)

    SiliaPrep SPE Cartridges Silica (SPE-R10030B)

    SiliaPrep Silica is the most polar sorbent presenting a slightly acidic character used to extract various compounds from non-polar solvents using hydrogen bonding. Efficient sorbent for the separation of analytes with similar structures and for removing the baseline noise from organic samples.

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