Fluoroquinolones
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Matrix
Milk
Analytes
Norfloxacin, Ofloxacin, Ciprofloxacin, Pefloxacin, Lomefloxacin, Danofloxacin, Enrofloxacin, Sarafloxacin, Difloxacin, Oxolinic Acid, Flumequine
SPE Cartridge
SiliaPrepX DVB
60 mg / 3 mL
SPE-P0001-03BB
Sample Pretreatment before Loading
Add 15 mL of Trichloroacetic Acid / Acetonitrile (10:90) to 1 g of milk.
Mix / vortex.
Centrifugate at 5,000 rpm at 4°C for 10 minutes.
Conditionning
3 mL of Ethyl Acetate
Equilibration
3 mL of Water
Loading
Pretreated sample
Washing
3 mL of Methanol / Water (10:90)
Dry the cartridge
Elution
3 mL of Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with mobile phase and identification by HPLC.
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SiliaPrepX Polymeric SPE Cartridges, DVB, 85 µm, 60 Å (SPE-P0001)
SiliaPrepX DVB is a polystyrene-divinylbenzene copolymer presenting a high hydrophobicity used as reversed-phase for extraction of neutral, acidic and basic compounds in viscous matrices.