Fluoroquinolones

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Matrix

Milk

Analytes

Norfloxacin, Ofloxacin, Ciprofloxacin, Pefloxacin, Lomefloxacin, Danofloxacin, Enrofloxacin, Sarafloxacin, Difloxacin, Oxolinic Acid, Flumequine

SPE Cartridge

SiliaPrepX DVB
60 mg / 3 mL
SPE-P0001-03BB

Sample Pretreatment before Loading

Add 15 mL of Trichloroacetic Acid / Acetonitrile (10:90) to 1 g of milk.
Mix / vortex.
Centrifugate at 5,000 rpm at 4°C for 10 minutes.

Conditionning

3 mL of Ethyl Acetate

Equilibration

3 mL of Water

Loading

Pretreated sample

Washing

3 mL of Methanol / Water (10:90)
Dry the cartridge

Elution

3 mL of Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with mobile phase and identification by HPLC.