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Hormones

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Matrix

Serum

Analytes

Aldosterone, Cortisol, Cortisone, Corticosterone, 11-Deoxycortisol, β-Estradiol, Testosterone, 11-Deoxycorticosterone, Androstenedione, Estrone, 17αOH Progesterone, DHT, Progesterone

SPE Cartridge

SiliaPrepX DVB
30 mg / 1 mL
SPE-P0001-01AA

Sample Pretreatment before Loading

Add 400 μL of a 0.5 % Formic Acid solution to 100 μL of serum. Vortex, centrifugate, discard cellular debris.

Conditionning

1 mL of 0.5 % Formic Acid in Methanol

Equilibration

1 mL of 0.5 % Formic Acid in Water

Loading

Pretreated sample

Washing

1 mL of Methanol / Water (30:70)
Dry the cartridge

Elution

2 x 0.25 mL of Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with Methanol / Water (50:50) and quantification by LC/MS/MS.

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  1. SiliaPrepX DVB Polymeric SPE Cartridges, 85 µm, 60 Å (SPE-P0001)

    SiliaPrepX DVB Polymeric SPE Cartridges, 85 µm, 60 Å (SPE-P0001)

    SiliaPrepX DVB is a polystyrene-divinylbenzene copolymer presenting a high hydrophobicity used as reversed-phase for extraction of neutral, acidic and basic compounds in viscous matrices.

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