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Melamine & Analogues

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Powdered infant milk


Melamine, Ammeline, Ammelide

SPE Cartridge

SiliaPrepX SCX
60 mg / 3 mL

Sample Pretreatment before Loading

Add 20 mL of Water to 1 g of powdered infant milk, vortex. Take 1 mL and add 2 mL of HCl (0.1 N).


3 mL of Methanol


3 mL of Water


Pretreated sample


3 mL of 2 % Formic Acid in Water
3 mL of Methanol / Acetonitrile (50:50)


3 mL of 5 % Ammonium Hydroxide in Methanol / Acetonitrile (50:50)

Further Treatment

Evaporation under Nitrogen, reconstitution with Methanol / Acetonitrile (50:50) and quantification by LC/MS.

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