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Melamine & Analogues
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Matrix
Powdered infant milk
Analytes
Melamine, Ammeline, Ammelide
SPE Cartridge
SiliaPrepX SCX
60 mg / 3 mL
SPE-P0005-03BB
Sample Pretreatment before Loading
Add 20 mL of Water to 1 g of powdered infant milk, vortex. Take 1 mL and add 2 mL of HCl (0.1 N).
Conditionning
3 mL of Methanol
Equilibration
3 mL of Water
Loading
Pretreated sample
Washing
3 mL of 2 % Formic Acid in Water
3 mL of Methanol / Acetonitrile (50:50)
Elution
3 mL of 5 % Ammonium Hydroxide in Methanol / Acetonitrile (50:50)
Further Treatment
Evaporation under Nitrogen, reconstitution with Methanol / Acetonitrile (50:50) and quantification by LC/MS.
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SiliaPrepX Polymeric SPE Cartridges, Tosic Acid (SCX), 85 µm, 60 Å (SPE-P0005)
SiliaPrepX SCX is a polystyrene-divinylbenzene copolymer functionalized by a strong cation exchanger presenting a high selectivity for acids (pKa 2 - 10). It is highly stable in organic solvents.