MMA & Succinic Acid
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Matrix
Plasma
Analytes
Methylmalonic Acid (MMA), Succinic Acid
SPE Cartridge
SiliaPrepX WAX
30 mg / 1 mL
SPE-P0020-01AA
Sample Pretreatment before Loading
Dilute 100 μL of plasma with 0.5 mL of 0.1 % Acetic Acid.
Conditionning
1 mL of Methanol
Equilibration
1 mL of 0.1 % Acetic Acid in Water
Loading
Pretreated sample
Washing
0.5 mL of Methanol / Water (50:50)
Dry the cartridge
Elution
2 x 0.6 mL of 2 % Ammonium Hydroxide in Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.
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SiliaPrepX Polymeric SPE Cartridges, Amine (WAX), 85 µm, 60 Å (SPE-P0020)
SiliaPrepX WAX is a polystyrene-divinylbenzene copolymer functionalized by a weak anion exchanger used to catch and release strong acidic compounds (pKa <2). It is highly stable in organic solvents.