MMA & Succinic Acid

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Matrix

Plasma

Analytes

Methylmalonic Acid (MMA), Succinic Acid

SPE Cartridge

SiliaPrepX WAX
30 mg / 1 mL
SPE-P0020-01AA

Sample Pretreatment before Loading

Dilute 100 μL of plasma with 0.5 mL of 0.1 % Acetic Acid.

Conditionning

1 mL of Methanol

Equilibration

1 mL of 0.1 % Acetic Acid in Water

Loading

Pretreated sample

Washing

0.5 mL of Methanol / Water (50:50)
Dry the cartridge

Elution

2 x 0.6 mL of 2 % Ammonium Hydroxide in Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.