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Opiates
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Matrix
Urine
Analytes
Morphine, Codeine, Hydromorphone, Norcodeine, Hydrocodone, Oxycodone, Oxymorphone
SPE Cartridge
SiliaPrepX SCX
30 mg / 3 mL
SPE-P0005-03AA
Sample Pretreatment before Loading
Add 125 µL of concentrated HCl to 0.5 mL of urine.
Heat at 95°C for 1.5 hour.
Cool, add 2 mL of Sodium Acetate buffer (0.1 M, pH 4.5).
Neutralize with 250 µL of Potassium Hydroxide (7 N), vortex.
pH should be inferior to 6.0.
Centrifugate at 6,000 rpm for 20 minutes.
Conditionning
0.5 mL of Methanol
Equilibration
1 mL of Water
Loading
Pretreated sample
Washing
1 mL of 2 % Formic Acid
1 mL of Methanol
Dry the cartridge
Elution
2 mL of Ammonium Hydroxide / Methanol (20:100)
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Methanol / Water (5:95) and quantification by LC/MS/MS.
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SiliaPrepX Polymeric SPE Cartridges, Tosic Acid (SCX), 85 µm, 60 Å (SPE-P0005)
SiliaPrepX SCX is a polystyrene-divinylbenzene copolymer functionalized by a strong cation exchanger presenting a high selectivity for acids (pKa 2 - 10). It is highly stable in organic solvents.