Opiates

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Matrix

Urine

Analytes

Morphine, Codeine, Hydromorphone, Norcodeine, Hydrocodone, Oxycodone, Oxymorphone

SPE Cartridge

SiliaPrepX SCX
30 mg / 3 mL
SPE-P0005-03AA

Sample Pretreatment before Loading

Add 125 µL of concentrated HCl to 0.5 mL of urine.
Heat at 95°C for 1.5 hour.
Cool, add 2 mL of Sodium Acetate buffer (0.1 M, pH 4.5).
Neutralize with 250 µL of Potassium Hydroxide (7 N), vortex.
pH should be inferior to 6.0.
Centrifugate at 6,000 rpm for 20 minutes.

Conditionning

0.5 mL of Methanol

Equilibration

1 mL of Water

Loading

Pretreated sample

Washing

1 mL of 2 % Formic Acid
1 mL of Methanol
Dry the cartridge

Elution

2 mL of Ammonium Hydroxide / Methanol (20:100)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Methanol / Water (5:95) and quantification by LC/MS/MS.