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Polycyclic aromatic hydrocarbons (PAHs)

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Matrix

Chocolate

Analytes

Naphthalene, Acenaphthylene, Acenaphthene, Fluorene, Phenanthrene, Anthracene, Fluoranthene, Pyrene, Benzo[c]fluorene, Benzo[a]anthracene, Chrysene, Cyclopental[cd]pyrene, Triphenylene, 5-methyl-Chrysene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[j]fluoranthene, Benzo[a]pyrene, Dibenz[a,h]anthracene, Indeno[1,2,3-cd]pyrene, Benzo[ghi]perylene, Dibenzo[a,l]pyrene, Dibenzo[a,e]pyrene, Dibenzo[a,i]pyrene, Dibenzo[a,h]pyrene

SPE Cartridge

SiliaPrep Silica
1 g / 6 mL
SPE-R10030B-06S

Sample Pretreatment before Loading

Grind 1 g of chocolate with 10 mL of Methanol to extract fats and coca butter.
Evaporate Methanol under Nitrogen.
Add 10 mL of Water then 5 mL of n-Pentane and proceed to liquid-liquid extraction.
Repeat the extraction with another 5 mL of n-Pentane.
Combine the two portions of 5 mL of n-Pentane, evaporate to 2 mL under Nitrogen.

Conditionning

6 mL of Methanol

Equilibration

6 mL of THF
6 mL of n-Pentane

Loading

Pretreated sample

Washing

3 mL of n-Pentane

Elution

2 x 5 mL of Methanol / THF (10:90)

Further Treatment

Evaporation under Nitrogen, reconstitution with Methanol and quantification by GC/MS.

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  1. SiliaPrep SPE Cartridges Silica (SPE-R10030B)

    SiliaPrep SPE Cartridges, Silica, 40 - 63 µm, 60 Å (SPE-R10030B)

    SiliaPrep Silica is the most polar sorbent presenting a slightly acidic character used to extract various compounds from non-polar solvents using hydrogen bonding. Efficient sorbent for the separation of analytes with similar structures and for removing the baseline noise from organic samples.

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