Parabens

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Matrix

Cosmetics (toothpaste)

Analytes

Methyl Paraben, Propyl Paraben

SPE Cartridge

SiliaPrep C8
500 mg / 3 mL
SPE-R31030B-03P

Sample Pretreatment before Loading

Add 10 mL of Methanol to 1 g of toothpaste, vortex, centrifugate.
Take 100 µL of mixture and dilute to 2 mL with Methanol.

Conditionning

3 mL of Methanol

Equilibration

3 mL of Water

Loading

Pretreated sample

Washing

3 mL of Water
Dry the cartridge

Elution

1 mL of Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with Water / Methanol (62:38) and identification by HPLC.