Parabens
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Matrix
Cosmetics (toothpaste)
Analytes
Methyl Paraben, Propyl Paraben
SPE Cartridge
SiliaPrep C8
500 mg / 3 mL
SPE-R31030B-03P
Sample Pretreatment before Loading
Add 10 mL of Methanol to 1 g of toothpaste, vortex, centrifugate.
Take 100 µL of mixture and dilute to 2 mL with Methanol.
Conditionning
3 mL of Methanol
Equilibration
3 mL of Water
Loading
Pretreated sample
Washing
3 mL of Water
Dry the cartridge
Elution
1 mL of Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with Water / Methanol (62:38) and identification by HPLC.
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SiliaPrep C8 is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds. SiliaPrep C8 is more selective than the C18 phase for compounds such as heavy PAH, vitamins D and oil & greasy compounds.
