Penicillins

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Matrix

Surface water

Analytes

Amoxicillin, Ampicillin, Penicillin G, Penicillin V, Oxacillin, Cloxacillin, Nafcillin, Dicloxacillin

SPE Cartridge

SiliaPrep C18 Plus
200 mg / 3 mL
SPE-R00830B-03G

Sample Pretreatment before Loading

Spike standards in 2 mL of water sample.

Conditionning

4 mL of Methanol

Equilibration

4 mL of Water

Loading

Pretreated sample

Washing

4 mL of 0.1 % Formic Acid in Water

Elution

2 x 2 mL of Acetonitrile

Further Treatment

Evaporation under Nitrogen, reconstitution with Water and quantification by LC/MS/MS.