Peptides
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Matrix
Plasma, serum
Analytes
Angiotensin I, Angiotensin II, Angiotensin III
SPE Cartridge
SiliaPrepX HLB
30 mg / 1 mL
SPE-P0002-01AA
Sample Pretreatment before Loading
Add 300 μL of Water to 300 μL of serum. Mix.
Conditionning
1 mL of Methanol
Equilibration
1 mL of Water
Loading
Pretreated sample
Washing
1 mL of Methanol / 0.1 % TFA in Water (10:90)
Dry the cartridge
Elution
1 mL of 2 % Ammonium Hydroxide in Methanol / Water (50:50)
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % TFA in Water and identification by HPLC.
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SiliaPrepX HLB Polymeric SPE Cartridges, 40 µm, 110 Å (SPE-P0002)
SiliaPrepX HLB is a wettable copolymer presenting a Hydrophilic-Lipophilic Balance (HLB) permitting a strong retention for neutral, acidic and basic compounds and a high stability in organic solvents.