Peptides

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Matrix

Plasma, serum

Analytes

Angiotensin I, Angiotensin II, Angiotensin III

SPE Cartridge

SiliaPrepX HLB
30 mg / 1 mL
SPE-P0002-01AA

Sample Pretreatment before Loading

Add 300 μL of Water to 300 μL of serum. Mix.

Conditionning

1 mL of Methanol

Equilibration

1 mL of Water

Loading

Pretreated sample

Washing

1 mL of Methanol / 0.1 % TFA in Water (10:90)
Dry the cartridge

Elution

1 mL of 2 % Ammonium Hydroxide in Methanol / Water (50:50)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % TFA in Water and identification by HPLC.