Peptides

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Matrix

Plasma, serum

Analytes

Oxytocin, Vasopressin

SPE Cartridge

SiliaPrepX WCX
30 mg / 2 mL
96W-P0015-AA

Sample Pretreatment before Loading

Add 300 μL of 4 % Phosphoric Acid to 300 μL of plasma / serum. Mix.

Conditionning

1 mL of Methanol

Equilibration

1 mL of Water

Loading

Pretreated sample

Washing

1 mL of 4 % Phosphoric Acid
1 mL of Methanol / Water (30:70)
Dry wells

Elution

2 x 0.75 mL of 1 % TFA in Acetonitrile / Methanol (50:50)

Further Treatment

Evaporation under Nitrogen, reconstitution with 1 % TFA in 0.1 % Formic Acid / 50 % Methanol in Acetonitrile with 0.1 % Formic Acid (80:20) and quantification by LC/MS/MS.