Peptides
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Matrix
Plasma, serum
Analytes
Oxytocin, Vasopressin
SPE Cartridge
SiliaPrepX WCX
30 mg / 2 mL
96W-P0015-AA
Sample Pretreatment before Loading
Add 300 μL of 4 % Phosphoric Acid to 300 μL of plasma / serum. Mix.
Conditionning
1 mL of Methanol
Equilibration
1 mL of Water
Loading
Pretreated sample
Washing
1 mL of 4 % Phosphoric Acid
1 mL of Methanol / Water (30:70)
Dry wells
Elution
2 x 0.75 mL of 1 % TFA in Acetonitrile / Methanol (50:50)
Further Treatment
Evaporation under Nitrogen, reconstitution with 1 % TFA in 0.1 % Formic Acid / 50 % Methanol in Acetonitrile with 0.1 % Formic Acid (80:20) and quantification by LC/MS/MS.
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