Per- and Polyfluoroalkyl Substances (PFASs)

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Matrix

Drinking water

Analytes

PFBA, PFPeA, PFHxA, PFHpA, PFOA, PFNA, PFDA, PFUdA, PFDoA, PFTrDA, PFTeDA, PFBS, PFPeS, PFHxS, PFHpS, PFOS, PFNS, PFDS, FOSA, 4:2 FTS, 6:2 FTS, 8:2 FTS, FOSAA, N-MeFOSAA, N-EtFOSAA, PFHxPA

SPE Cartridge

SiliaPrep (WAX)
200 mg / 6 mL
SPE-R52030B-06G

Sample Pretreatment before Loading

Add 3 mL of Phosphate buffer (100 mM, pH 6.0) to 2 mL of blood / plasma / serum / urine (or 1 g of tissue homogenate).
Mix / vortex.
Adjust pH to 6.0 ± 0.5 with Sodium Phosphate (100 mM).
Centrifugate at 2,000 rpm for 10 minutes and discard cellular debris.

Conditionning

3 mL of Methanol

Equilibration

5 mL of Phosphate buffer (100 mM, pH 7.0)

Loading

Pretreated sample
Dry the cartridge

Washing

No washing required

Elution

6 mL of 1 % Ammonium Hydroxide in Methanol

Further Treatment

Evaporation down to 1 mL under Nitrogen and quantification by LC/MS/MS.