Polychlorinated Biphenyls (PCB)

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Matrix

Surface water

Analytes

2-Chlorobiphenyl, 4-Chlorobiphenyl, 2,4ʹ-Dichlorobiphenyl, 2,2ʹ,5-Trichlorobiphenyl, 2,4,4ʹ-Trichlorobiphenyl, 2,2ʹ,3,5ʹ-Tetrachlorobiphenyl, 2,2ʹ,5,5ʹ-Tetrachlorobiphenyl, 2,3ʹ,4ʹ,5-Tetrachloroobiphenyl, 2,3,3ʹ,4ʹ,6-Pentachlorobiphenyl, 2,3ʹ,4,4ʹ,5-Pentachlorobiphenyl, 2,2ʹ,3,4,4ʹ,5ʹ-Hexachlorobiphenyl, 2,2ʹ,3,4ʹ,5ʹ,6-Hexachlorobiphenyl, 2,2ʹ,4,4ʹ,5,5ʹ- Hexachlorobiphenyl, 2,2ʹ,3,4,4ʹ,5,5ʹ-Heptachlorobiphenyl

SPE Cartridge

SiliaPrep C18 Plus
1.5 g / 6 mL
SPE-R00830B-06T

Sample Pretreatment before Loading

Add 0.1 g of L-Ascorbic Acid, 0.35 g of Ethylenediamine Tetra Acetic Acid Trisodium salt and 9.4 g of Potassium Citrate Monobasic to 1 L of surface water.

Conditionning

5 mL of Ethyl Acetate / Dichloromethane (50:50)
Dry the cartridge
10 mL of Methanol

Equilibration

6 mL of Water

Loading

Pretreated sample
Dry the cartridge

Washing

No washing required

Elution

5 mL of Ethyl Acetate
5 mL of Dichloromethane

Further Treatment

Dry fraction with Sodium Sulfate, evaporation down to 1 mL under Nitrogen and quantification by LC/MS/MS.