Sulfonamides

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Matrix

Honey

Analytes

Sulfanilamide, Sulfathiazole, Sulfamerazine, Sulfamethoxazole, Sulfaquinoxaline

SPE Cartridge

SiliaPrepX SCX
60 mg / 3 mL
SPE-P0005-03BB

Sample Pretreatment before Loading

Add 1 mL of Hydrochloric Acid (2 M) to 1 g of honey, sonicate for 30 minutes and dilute to 5 mL with Citric Acid (300 mM).

Conditionning

2 mL of Methanol

Equilibration

2 mL of Water

Loading

Pretreated sample

Washing

2 x 2 mL of Water
2 x 2 mL of Methanol / Acetonitrile (50:50)
Dry the cartridge

Elution

2 mL of 2 % Ammonium Hydroxide in Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water / Acetonitrile (90:10) and quantification by LC/MS/MS.