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Sulfonamides
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Matrix
Honey
Analytes
Sulfanilamide, Sulfathiazole, Sulfamerazine, Sulfamethoxazole, Sulfaquinoxaline
SPE Cartridge
SiliaPrepX SCX
60 mg / 3 mL
SPE-P0005-03BB
Sample Pretreatment before Loading
Add 1 mL of Hydrochloric Acid (2 M) to 1 g of honey, sonicate for 30 minutes and dilute to 5 mL with Citric Acid (300 mM).
Conditionning
2 mL of Methanol
Equilibration
2 mL of Water
Loading
Pretreated sample
Washing
2 x 2 mL of Water
2 x 2 mL of Methanol / Acetonitrile (50:50)
Dry the cartridge
Elution
2 mL of 2 % Ammonium Hydroxide in Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water / Acetonitrile (90:10) and quantification by LC/MS/MS.
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SiliaPrepX Polymeric SPE Cartridges, Tosic Acid (SCX), 85 µm, 60 Å (SPE-P0005)
SiliaPrepX SCX is a polystyrene-divinylbenzene copolymer functionalized by a strong cation exchanger presenting a high selectivity for acids (pKa 2 - 10). It is highly stable in organic solvents.