Sympathomimetic Amines
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Matrix
Biological fluids (blood, plasma, serum, urine or tissue)
Analytes
Phenylpropanolamine, Ephedrine, Phentermine, Diethylproprion, Pheniramine, Doxylamine, Chlorpheniramine, Brompheniramine, Diphenhydramine, Amphetamine, Methamphetamine, MDA, MDMA, Pseudoephedrine, Phenylephrine
SPE Cartridge
SiliaPrep CleanDRUG
200 mg / 6 mL
SPEC-R651230B-06G
Sample Pretreatment before Loading
Add 3 mL of Phosphate buffer (100 mM, pH 6.0) to 2 mL of blood / plasma / serum / urine (or 1 g of tissue homogenate).
Mix / vortex.
Adjust pH to 6.0 ± 0.5 with Sodium Phosphate (100 mM).
Centrifugate at 2,000 rpm for 10 minutes and discard cellular debris.
Conditionning
3 mL of Methanol
Equilibration
3 mL of Water
3 mL of Phosphate buffer (100 mM, pH 6.0)
Loading
Pretreated sample
Washing
3 mL of Water
3 mL of Acetic Acid (100 mM)
3 mL of Methanol
Dry the cartridge
Elution
3 mL of Dichloromethane / Isopropanol / Ammonium Hydroxide (78:20:2, pH 11 ‑ 12)
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.
Or evaporation under Nitrogen, fluoroacylation with PFPA, evaporation under Nitrogen, reconstitution with Ethyl Acetate and quantification by GC/MS.
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SiliaPrep SPE Cartridges, CleanDRUG (SPEC-R651230B)
SiliaPrep CleanDRUG phase has been optimized for drugs of abuse applications