Sympathomimetic Amines

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Matrix

Biological fluids (blood, plasma, serum, urine or tissue)

Analytes

Phenylpropanolamine, Ephedrine, Phentermine, Diethylproprion, Pheniramine, Doxylamine, Chlorpheniramine, Brompheniramine, Diphenhydramine, Amphetamine, Methamphetamine, MDA, MDMA, Pseudoephedrine, Phenylephrine

SPE Cartridge

SiliaPrep CleanDRUG
200 mg / 6 mL
SPEC-R651230B-06G

Sample Pretreatment before Loading

Add 3 mL of Phosphate buffer (100 mM, pH 6.0) to 2 mL of blood / plasma / serum / urine (or 1 g of tissue homogenate).
Mix / vortex.
Adjust pH to 6.0 ± 0.5 with Sodium Phosphate (100 mM).
Centrifugate at 2,000 rpm for 10 minutes and discard cellular debris.

Conditionning

3 mL of Methanol

Equilibration

3 mL of Water
3 mL of Phosphate buffer (100 mM, pH 6.0)

Loading

Pretreated sample

Washing

3 mL of Water
3 mL of Acetic Acid (100 mM)
3 mL of Methanol
Dry the cartridge

Elution

3 mL of Dichloromethane / Isopropanol / Ammonium Hydroxide (78:20:2, pH 11 ‑ 12)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.
Or evaporation under Nitrogen, fluoroacylation with PFPA, evaporation under Nitrogen, reconstitution with Ethyl Acetate and quantification by GC/MS.