Follow SiliCycle on Facebook Follow SiliCycle on Twitter Follow SiliCycle on LinkedIn Watch our videos on Youtube

Sympathomimetic Amines

> back to Suggested SPE Protocols

Matrix

Biological fluids (blood, plasma, serum, urine or tissue)

Analytes

Phenylpropanolamine, Ephedrine, Phentermine, Diethylproprion, Pheniramine, Doxylamine, Chlorpheniramine, Brompheniramine, Diphenhydramine, Amphetamine, Methamphetamine, MDA, MDMA, Pseudoephedrine, Phenylephrine

SPE Cartridge

SiliaPrep CleanDRUG
200 mg / 6 mL
SPEC-R651230B-06G

Sample Pretreatment before Loading

Add 3 mL of Phosphate buffer (100 mM, pH 6.0) to 2 mL of blood / plasma / serum / urine (or 1 g of tissue homogenate).
Mix / vortex.
Adjust pH to 6.0 ± 0.5 with Sodium Phosphate (100 mM).
Centrifugate at 2,000 rpm for 10 minutes and discard cellular debris.

Conditionning

3 mL of Methanol

Equilibration

3 mL of Water
3 mL of Phosphate buffer (100 mM, pH 6.0)

Loading

Pretreated sample

Washing

3 mL of Water
3 mL of Acetic Acid (100 mM)
3 mL of Methanol
Dry the cartridge

Elution

3 mL of Dichloromethane / Isopropanol / Ammonium Hydroxide (78:20:2, pH 11 ‑ 12)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.
Or evaporation under Nitrogen, fluoroacylation with PFPA, evaporation under Nitrogen, reconstitution with Ethyl Acetate and quantification by GC/MS.

1 Item(s)

Set Descending Direction
per page

List  Grid 

  1. SiliaPrep SPE Cartridges CleanDRUG (SPEC-R651230B)

    SiliaPrep SPE Cartridges CleanDRUG (SPEC-R651230B)

    SiliaPrep CleanDRUG phase has been optimized for drugs of abuse applications

1 Item(s)

Set Descending Direction
per page

List  Grid