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Sympathomimetic Amines

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Biological fluids (blood, plasma, serum, urine or tissue)


Phenylpropanolamine, Ephedrine, Phentermine, Diethylproprion, Pheniramine, Doxylamine, Chlorpheniramine, Brompheniramine, Diphenhydramine, Amphetamine, Methamphetamine, MDA, MDMA, Pseudoephedrine, Phenylephrine

SPE Cartridge

SiliaPrep CleanDRUG
200 mg / 6 mL

Sample Pretreatment before Loading

Add 3 mL of Phosphate buffer (100 mM, pH 6.0) to 2 mL of blood / plasma / serum / urine (or 1 g of tissue homogenate).
Mix / vortex.
Adjust pH to 6.0 ± 0.5 with Sodium Phosphate (100 mM).
Centrifugate at 2,000 rpm for 10 minutes and discard cellular debris.


3 mL of Methanol


3 mL of Water
3 mL of Phosphate buffer (100 mM, pH 6.0)


Pretreated sample


3 mL of Water
3 mL of Acetic Acid (100 mM)
3 mL of Methanol
Dry the cartridge


3 mL of Dichloromethane / Isopropanol / Ammonium Hydroxide (78:20:2, pH 11 ‑ 12)

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.
Or evaporation under Nitrogen, fluoroacylation with PFPA, evaporation under Nitrogen, reconstitution with Ethyl Acetate and quantification by GC/MS.

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  1. SiliaPrep SPE Cartridges CleanDRUG (SPEC-R651230B)

    SiliaPrep SPE Cartridges, CleanDRUG (SPEC-R651230B)

    SiliaPrep CleanDRUG phase has been optimized for drugs of abuse applications

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