Synthetic Dyes & Metabolites

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Matrix

Seafood

Analytes

Malachite Green, Leucomalachite Green, Crystal Violet, Leucocrystal Violet, Nile Blue, Azure B, Methylene Blue, Brilliant Green, Victoria Blue

SPE Cartridge

SiliaPrep CleanDRUG
200 mg / 6 mL
SPEC-R651230B-06G

Sample Pretreatment before Loading

Add 10 mL of 1 % Formic Acid in Acetonitrile and 1 mL of Ascorbic Acid (1 M, used as antioxidant) to 2 g of sample.
Vortex for 15 minutes, centrifugate for 10 minutes at 3,000 rpm and 4°C.
Take the supernatant and add 20 mL of McIlvaine's buffer (0.1 M, pH 3.5).
Vortex and centrifugate again.

Conditionning

3 mL of Methanol

Equilibration

3 mL of Water
1 mL of McIlvaine's buffer (0.1 M, pH 3.5)

Loading

Pretreated sample (supernatant)

Washing

3 mL of 0.1 % Formic Acid in Water
3 mL of 0.1 % Formic Acid in Methanol
Dry the cartridge

Elution

4 mL of 1 % Triethylamine and 0.5 % Formic Acid in Methanol

Further Treatment

Quantification by LC/MS/MS.