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Oral fluid


Cotinine, Anabasine, Nicotine

Well Plate

SiliaPrepX SCX
30 mg / 2 mL

Sample Pretreatment before Loading

Collect oral fluid according to instructions on the device, place sample in adequate buffer solution and centrifugate. Add 1 mL of 1 % Formic Acid to 0.5 mL of supernatant.


1 mL of Methanol


1 mL of Water


Pretreated sample


1 mL of Water
1 mL of Acetone / Water (50:50)
Dry wells


2 x 500 μL of Ethyl acetate / Isopropanol / Ammonium Hydroxide (70:20:10)

Further Treatment

Evaporation under Nitrogen, reconstitution with Ammonium Bicarbonate (20 mM, pH 8.2) / Methanol (90:10) and quantification by LC/MS/MS.

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  1. SiliaPrepX Polymeric Well Plates, SCX, 85 µm, 60 Å (96W-P0005)

    SiliaPrepX Polymeric Well Plates, Tosic Acid (SCX), 85 µm, 60 Å (96W-P0005)

    SiliaPrepX SCX is a polystyrene-divinylbenzene copolymer functionalized by a strong cation exchanger presenting a high selectivity for acids (pKa 2 - 10). It is highly stable in organic solvents.

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