Topical anesthetics

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Matrix

Serum

Analytes

Benzocaine, Procaine, Mepivacaine

SPE Cartridge

SiliaPrep C8
500 mg / 6 mL
SPE-R31030B-06P

Sample Pretreatment before Loading

Add 500 µL of internal standard solution to 500 µL of serum. Vortex.

Conditionning

6 mL of Methanol

Equilibration

6 mL of Water

Loading

Pretreated sample

Washing

6 mL of Water / Methanol (75:25)
Dry the cartridge

Elution

2 mL of Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with Chloroform and identification by GC.