Topical anesthetics
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Matrix
Serum
Analytes
Benzocaine, Procaine, Mepivacaine
SPE Cartridge
SiliaPrep C8
500 mg / 6 mL
SPE-R31030B-06P
Sample Pretreatment before Loading
Add 500 µL of internal standard solution to 500 µL of serum. Vortex.
Conditionning
6 mL of Methanol
Equilibration
6 mL of Water
Loading
Pretreated sample
Washing
6 mL of Water / Methanol (75:25)
Dry the cartridge
Elution
2 mL of Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with Chloroform and identification by GC.
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SiliaPrep SPE Cartridges C8 (SPE-R31030B)
SiliaPrep C8 is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds. SiliaPrep C8 is more selective than the C18 phase for compounds such as heavy PAH, vitamins D and oil & greasy compounds.