Triazoles
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Matrix
Plasma
Analytes
Fluconazole, Voriconazole, Posaconazole, Ketoconazole, Hydroxy-itraconazole, Itraconazole
SPE Cartridge
SiliaPrepX DVB
30 mg / 1 mL
SPE-P0001-01AA
Sample Pretreatment before Loading
Dilute 300 μL of plasma to 1 mL, adjust pH to 2.0.
Conditionning
1 mL of Methanol
Equilibration
1 mL of Water
Loading
Pretreated sample
Washing
1 mL of 1 % Ammonium Hydroxide in Water
1 mL of Water / Methanol (70:30)
Elution
1 mL of Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with Water / Methanol (50:50) and identification by HPLC.
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SiliaPrepX Polymeric SPE Cartridges, DVB, 85 µm, 60 Å (SPE-P0001)
SiliaPrepX DVB is a polystyrene-divinylbenzene copolymer presenting a high hydrophobicity used as reversed-phase for extraction of neutral, acidic and basic compounds in viscous matrices.