Triazoles

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Matrix

Plasma

Analytes

Fluconazole, Voriconazole, Posaconazole, Ketoconazole, Hydroxy-itraconazole, Itraconazole

SPE Cartridge

SiliaPrepX DVB
30 mg / 1 mL
SPE-P0001-01AA

Sample Pretreatment before Loading

Dilute 300 μL of plasma to 1 mL, adjust pH to 2.0.

Conditionning

1 mL of Methanol

Equilibration

1 mL of Water

Loading

Pretreated sample

Washing

1 mL of 1 % Ammonium Hydroxide in Water
1 mL of Water / Methanol (70:30)

Elution

1 mL of Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with Water / Methanol (50:50) and identification by HPLC.