Urinary Catecholamines

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Matrix

Urine

Analytes

Dopamine, Norepinephrine, Epinephrine, Normetanephrine, Metanephrine

SPE Well Plate

SiliaPrepX WCX
10 mg / 2 mL
96W-P0015-1A

Sample Pretreatment before Loading

Dilute 75 μL of urine with 150 μL of Ammonium Acetate (250 mM), mix.

Conditionning

500 μL of Methanol

Equilibration

500 μL of Ammonium Acetate (10 mM)

Loading

150 μL of pretreated sample

Washing

500 μL of Ammonium Acetate (10 mM)
500 μL of Isopropanol

Elution

125 μL of 0.1 % Formic Acid / Isopropanol (85:15)

Further Treatment

Quantification by LC/MS/MS.