Urinary Catecholamines
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Matrix
Urine
Analytes
Dopamine, Norepinephrine, Epinephrine, Normetanephrine, Metanephrine
SPE Well Plate
SiliaPrepX WCX
10 mg / 2 mL
96W-P0015-1A
Sample Pretreatment before Loading
Dilute 75 μL of urine with 150 μL of Ammonium Acetate (250 mM), mix.
Conditionning
500 μL of Methanol
Equilibration
500 μL of Ammonium Acetate (10 mM)
Loading
150 μL of pretreated sample
Washing
500 μL of Ammonium Acetate (10 mM)
500 μL of Isopropanol
Elution
125 μL of 0.1 % Formic Acid / Isopropanol (85:15)
Further Treatment
Quantification by LC/MS/MS.
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SiliaPrepX Polymeric Well Plates, Carboxylic Acid (WCX), 85 µm, 60 Å (96W-P0015)
SiliaPrepX WCX is a polystyrene-divinylbenzene copolymer functionalized by a weak cation exchanger used to catch and release strong basic compounds (pKa >10). It is highly stable in organic solvents.