Vitamin B3 & Metabolites
> back to Suggested SPE Protocols
Matrix
Plasma, serum
Analytes
Nicotinic Acid, Nicotinuric Acid, Niacinamide
SPE Cartridge
SiliaPrep SCX nec
50 mg / 1 mL
SPE-R60430B-01B
Sample Pretreatment before Loading
Add 150 μL of 2 % aqueous Acetic Acid to 50 μL of serum, mix.
Conditionning
1 mL of Methanol
Equilibration
1 mL of 2 % Acetic Acid
Loading
Pretreated sample
Washing
2 x 1 mL of Water / Methanol / Acetic Acid (68:30:2)
2 x 1 mL of 2 % Acetic Acid in Methanol
Elution
2 x 400 μL of 5 % Ammonium Hydroxide in Methanol
Further Treatment
Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid and quantification by LC/MS/MS.
-
SiliaPrep SPE Cartridges Tosic Acid (SCX) nec (SPE-R60430B)
SiliaPrep Tosic Acid is a strong cation exchanger and maintains a negative charge throughtout the pH scale. The most common use is a strong cation exchanger for the amine and basics molecules "catch and release" purification technique in SPE cartridges.