Vitamin B7

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Matrix

Serum

Analytes

Biotin (Vitamin B7)

SPE Cartridge

SiliaPrepX SAX
100 mg / 3 mL
SPE-P0010-03C

Sample Pretreatment before Loading

Add 750 μL of 0.1 % Ammonium Hydroxide in Water to 250 μL of serum. Vortex.

Conditionning

3 mL of Methanol

Equilibration

3 mL of 0.1 % Ammonium Hydroxide in Water

Loading

Pretreated sample

Washing

3 mL of 0.1 % Ammonium Hydroxide in Water
3 mL of Methanol

Elution

2 x 1 mL of 2 % Formic Acid in Methanol

Further Treatment

Evaporation under Nitrogen, reconstitution with 0.1 % Formic Acid in Water and quantification by LC/MS/MS.