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Difference between TLG-R10014B-323 and TLG-R10014BK-323 SiliaPlate TLC Plates

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SiliCycle produces 2 types of Glass-Backed TLC Plates 20x20 cm, and they both have different sensitivities and areas of applications.

Description: SiliaPlate G, TLC - Glass-Backed, Hard Layer, 250 μm, 20x20 cm, F254 (25/box)
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Description: SiliaPlate G, TLC - Glass-Backed, Special Layer for KMnO4, 250 μm, 20x20 cm, F254 (25/box)
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Mean Particle Size: 10 – 12 μm
Pore Diameter: 60 Å
Surface Area: ≈ 500 m2/g
Layer Thickness: ≈ 250 μm
Mobile Phase Compatibility: All solvent combinations, organic and aqueous
Prolonged Storage: Keep dry
Shelf-Life: 1 year

SiliaPlate TLC Launch Notification

SiliCycle is very pleased to announce the launch of our new and improved TLC plate layers.
Those two new TLC were developped for increased performance in accordance with your analyte, mobile phase composition or preferred method of visualization. They have different sensitivities and areas of applications.

The difference between:

  • Glass-Backed, Hard Layer, 20x20 cm, TLC Plate
    (PN: TLG-R10014B-323)
  • Glass-Backed, Optimized Layer for KMnO4, 20x20 cm, TLC Plates
    (PN: TLG-R10014BK-323)
... is the binder chemistry:

  • TLG-R10014B-323‘s layer is polymeric: it has been added a small percentage of inorganic, hardening agent for a uniform and hard surface, smooth and dense, that will not crack, blister nor swell up.
    They were designed for maximum robustness of the binder: they are very easy to handle and to write on, as well as completely wettable.
    They are compatible with all solvents, yet, they might oxidize a bit faster when dipped into KMnO4 (fading in a few minutes from flashy purple to yellow ocher).
    Also, spots are a bit less definite when using CAM as a revelatory.
    Such binder also contains a higher percentage of fluorescent indicator for greater brilliance of spots and less background noice from the silica layer.
  • TLG-R10014BK-323‘s layer is gypsum (calcium sulfate), and do not contain the polymeric additive that provides the former plates a harder surface and ruggedness.
    This means that the layer is softer, so spots can be easily scrapped off from the glass support, and are particularily recommended for aggressive visualization methods (strong charring, CAM staining solution) or, if dipped into KMnO4, ought to remain bright-purple a longer period of time.


General View of Specificities & Characteristics to be Considered.

Hopefully this chart will help to discriminate and choose the right product in function of personal needs.

TLG-R10014B-323 TLG-R10014BK-323
UV Fluorescence (F254) Higher brightness
Less background noice from layer
Binder Sensitivity Stable in almost all organic solvents
Increased separation efficiency Resistant toward aggressive
visualization methods
Surface Layer Robust and rugged Easily scratched off
Water Tolerance ≥ 80 % Up to 40%
Polarity of Binder Medium polarity
Specific Surface (BET) ≈ 500 m2/g
Mean Pore Size 60 Å
Mean Pore Volume 0.75 mL/g
Distribution (Mean Particle Size) 5 – 17 μm [10 – 12 μm]
Layer Thickness ≈ 250 μm
Stain Compatibility
KMnO4 Compatible highly compatible
CAM Compatible
p-Anisaldehyde Compatible highly compatible
Ninhydrin highly compatible
Vanilin highly compatible

Please keep in mind that it is absolutely normal that you may have to adjust your mobile phase and / or separation conditions when switching from one plate to another. They have different binder, thus a slight different affinity and behaviour toward various molecules.

In its quest to always improve and provide the best possible products, SiliCycle is continuously reviewing how it can make a difference.

May you have any concerns or questions regarding our products, please do not hesitate to contact us.

It is always our pleasure to discuss chemistry issues with you.