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A New Era of Cell Culture Optimization

Current Cell Culture Situation

Mammalian cells are routinely cultured using a usual basal medium that is a bicarbonate-buffered isotonic aqueous solution, with high level of glucose supplemented with vitamins as well as essential amino acids. It is boosted with bovine calf serum (up to 10%) and customized with a set of purified growth factors and antibiotics.
It was recently highlighted that there is a severe lack in the composition of such cell culture medium. Effectively, they are deficient in one of a paramount cell component: essential fatty acids.


X-Pure OMEGA for Cell Culture

Omega ω-3 and ω-6 fatty acids are essential to maintain the homoeostasis of the human body. As we know, every cell needs essential fatty acids in order to function properly. Yet, there is only trace-levels of essential fatty acids in common fetal bovine serums and calf serums.
As a result, the lipid composition of cultured cell membranes is not physiological and contains mainly saturated fatty acids, also known as "bad fat". This has leaded scientists to study the effects of essential fatty acid supplementation on many cell types, and outcomes have been well documented in vivo and in vitro.
SiliCycle’s X-Pure Omega polyunsaturated fatty acids (PUFAs) are 99%+ pure; certified sterile and provided in convenient re-sealable twist cap vials. You now have access to X-Pure Culture Omega: a product pipeline specifically designed to be used as a cell culture supplement.


Why supplement with PUFAs?

Polyunsaturated Fatty Acids (PUFAs) influence vital aspects of the cellular physiology.

> see X-Pure Omega's applications


They should be readily available to cells in culture because of such strong influence:

PUFAs target of influence Benefits / Effects
Membrane fluidity [1] Incorporation of PUFAs in the cell membrane increase membrane fluidity and contributing to inter-cell signaling.
Mitochondria [2 - 4] PUFAs are involved in various mitochondrial processes including mitochondrial calcium homeostasis, gene expression, respiratory function, ROS production and mitochondrial apoptosis.
Protein folding, functionality and signaling [5][8, 9] PUFAs modulate of voltage-gated ion channels. Arachidonic acid (AA) was found to inhibit the function of whole-cell voltage-gated (VG) calcium currents, primarily in neurons and muscle cells.
Lipid rafts [6, 7] The incorporation of DHA-containing phospholipids in the cell membrane enhances the translocation of sterol into rafts. This modification protein localization and conformation and can have implications for cellular signaling events.
Endoplasmic reticulum trafficking [10, 11] PUFAs modulate phospholipase D (PLD) trafficking and activity in mast cells by preventing the migration of the PLD1 to the plasma membrane.
Inflammation [12] PUFAs are precursors of eicosanoids and are therefore implicated in inflammatory processes.
Oxidative stress (PMID: 22319624) PUFA scan reduces oxidative stress.

[1] Schumann J, It is all about fluidity: Fatty acids and macrophage phagocytosis, Eur J Pharmacol, 2016
doi: 10.1016/j.ejphar.2015.04.057

[2] Stanley WC, et al., Update on lipids and mitochondrial function: impact of dietary n-3 polyunsaturated fatty acids, Curr Opin Clin Nutr Metab Care, 2012
doi: 10.1097/MCO.0b013e32834fdaf7

[3] Putti R, et al., Skeletal Muscle Mitochondrial Bioenergetics and Morphology in High Fat Diet Induced Obesity and Insulin Resistance: Focus on Dietary Fat Source, Front Physiol, 2015
doi: 10.3389/fphys.2015.00426

[4] Rohrbach S, Effects of dietary polyunsaturated fatty acids on mitochondria, Curr Pharm Des, 2009
doi: 10.2174/138161209789909692

[5] Boland LM, et al., Polyunsaturated fatty acid modulation of voltage-gated ion channels, Cell Biochem Biophys, 2008
doi: 10.1007/s12013-008-9027-2

[6] Wassall SR, et al., Order from disorder, corralling cholesterol with chaotic lipids. The role of polyunsaturated lipids in membrane raft formation, Chem Phys Lipids, 2004
doi: 10.1016/j.chemphyslip.2004.09.007

[7] Shaikh SR, et al., How polyunsaturated fatty acids modify molecular organization in membranes: insight from NMR studies of model systems, Biochim Biophys Acta, 2015
doi: 10.1016/j.bbamem.2014.04.020

[8] Elinder F, et al., Actions and Mechanisms of Polyunsaturated Fatty Acids on Voltage-Gated Ion Channels, Front Physiol, 2017
doi: 10.3389/fphys.2017.00043

[9] Hou TY, et al., Omega-3 fatty acids, lipid rafts, and T cell signaling, Eur J Pharmacol, 2016
doi: 10.1016/j.ejphar.2015.03.091

[10] Lagace TA, et al., The role of phospholipids in the biological activity and structure of the endoplasmic reticulum, Biochim Biophys Acta, 2013

[11] Basiouni S, et al., The influence of polyunsaturated fatty acids on the phospholipase D isoforms trafficking and activity in mast cells, Int J Mol Sci, 2013
doi: 10.3390/ijms14059005

[12] Calder PC, Marine omega-3 fatty acids and inflammatory processes: Effects, mechanisms and clinical relevance, Biochim Biophys Acta, 2015
doi: 10.1016/j.bbalip.2014.08.010


Benefits of SiliCycle’s X-Pure Culture Omega PUFAs

SiliCycle’s X-Pure Omega grade Polyunsaturated Fatty Acids are:

  • 99%+ pure
  • Certified sterile
  • Packaged in convenient re-sealable twist cap vials
  • Purified with SiliCycle’s innovative technology
  • Provided with an unparalleled quality of service


Learn more about Culture Omega