HPLC Quiz
Do you have extensive knowledge regarding HPLC?
Take a few minutes to try our quiz! What score will you be able to get?
- About SiliCycle
-
Products
- Complete Range of Functionalized Silicas
- SiliCycle Scavenging Solutions
- E-PAK Purification Cartridges
- SiliaMetS - Metal Scavengers
-
SiliaBond - Functionalized Silica Gels
- General Portfolio
- SiliaBond Acids, Bases & Others
- SiliaBond Chromatographic Phases
-
SiliaBond for Organic Synthesis
- Selection Guide
- Acylation & Esterification Reactions
- Alkylation & Etherification Reactions
- Amide Coupling
- Deprotection of Aromatic Ethers
- Ether Formation
- Fmoc, Bsmoc Deprotection of Amino Acids
- Friedel-Crafts Alkylation
- Knoevenagel Condensation
- Michael Addition
- Nitro-Aldol (or Henry) Reactions
- Oxydations
- Reductive Aminations
- Urea Synthesis
- Other Reagents
- SiliaMetS Metal Scavengers
- SiliaBond Organic Scavengers
- SiliaCat - Heterogeneous Catalysts
- SiliaChrom Plus - HPLC
- SiliaFlash - Irregular Silica Gels
- SiliaSphere - Spherical Silica Gels
- SiliaPlate - TLC Plates
- SiliaSep - Flash Cartridges
- Sample Preparation Products
- Scientific Publications
- Services
- Industries
-
Resource Center
- Brochures
-
Metal and Organic Scavenging
-
Application Notes
- SM001 - Experimental Procedure Optimization for SiliaMetS Metal Scavengers
- SM002 - Comparing Silica and Polymer Supported Products
- SM005 - SiliaMetS Thiol and DMT Stability in Aqueous Media
- SM007 - Comparison Between SiliaMetS Metal Scavengers and Other Sorbents
- SM009 - Different Ways of Removing Rhodium
- SBS001 - Scavenging TPP and TPPO using SiliaBond Scavengers
- SB0012 - Scavenging Using SiliaBond Carbonate
- EP001 - Efficient Use of E-PAK Technology for the Removal of a Widely Used Suzuki Catalyst
- EP002 - Suzuki Catalysts Scavenging Study with Activated Carbon and SiliaMetS
- EP003 - Rhodium Scavenging Study with Activated Carbon and SiliaMetS
- EP004 - Efficient use of the E-PAK technology for removing iridium
- EP005 - Efficient method for the removal of nickel with E-PAK
- EP006 - Using E-PAK technology to remove chlorophyll in a biomass sample
- EP007 - Scaling up a Sonogashira Reaction with E-PAK
- EP008 - Screening and Efficient Removal of Ruthenium 3rd Generation Grubbs Catalyst
- Brochures
-
Case Studies
- CS-SM001 - A Practical Cleavage of Acetals Using SiliaMetS Thiol
- CS-SM002 - Screening of Various Metal Scavengers for Copper Removal in the Synthesis of a Bicyclo Derivative
- CS-SM003 - Scavenging of the Residual Buchwald-Hartwig Catalyst with SiliaMetS Thiol
- CS-SM004 - Efficiency of SiliaMetS Triamine for Copper Removal in the Preparation of Functionalized Phosphines by CuAA
- CS-SM005 - Enantiomer N-1 Substituted Tetrazole Prodrug Synthesis
- CS-SM007 - Improved Synthetic Route and Scale-Up of GSK973 using SiliaMetS Thiol for Pd Scavenging
- CS-SM008 - Separation of Palladium and Silver from E-Waste Leachate Using SiliaMetS Thiol
- CS-SM010 - Potential Antimalarial Drug Synthesis by Suzuki Coupling and Residual Palladium Clean-up with SiliaMetS Thiol
- CS-SM011 - Synthesis of a Library of Linear Bottlebrush Diblock Copolymers by Ruthenium Catalyzed ROMP
- CS-SM012 - Scale-Up of a Polonovski Reaction – Impact of Residual Palladium in Starting Material
- CS-SM013 - Solid Phase Extraction Materials for Removal of Silver Nanoparticles in Environmental Waters
- CS-SM014 - Scavenging of Copper from Amphiphilic Glycopeptides for the Creation of Supramolecular Hydrogels
- CS-SM015 - Separation of Various Metal Ions from High-Level Liquid Waste
- E-Books
- Posters
- White Papers
- Scavengers
-
Application Notes
- Chromatography and Purification
- Sample Preparation
- Analytical and Preparative Chromatography
- Organic Synthesis
- R&D Services
- Peptide Synthesis
- Posters
- Web Tools
- Customer Service
-
FAQ
- >> Flash Cartridges
-
>> Metal & Organic Scavengers
- What are metal scavengers?
- How to calculate how much metal scavenger to use?
- How to choose a metal scavenger?
- Why Choose Silica-Based vs Polymer-Based Scavengers?
- How to purify your API from organic contaminants?
- What is a supported scavenger?
- What quantity of scavenger should be used?
- At what temperature should the scavenger be used?
- What solvents should be used with our scavengers?
- At what pH should the scavenging in aqueous solution be performed?
- Reaction time vs scavenger quantity
- What is the thermal stability of the SiliaMetS?
- What is the molecular loading and how do you determine the molecular loading of a SiliaMetS metal scavenger?
-
>> HPLC
- What is HPLC?
- HPLC Columns Use Instructions
- What to do when backpressure increases?
- How do I determine the void volume in HPLC?
- Why should I use a guard column with my analytical or preparative column?
- Ideal injection volume vs column dimension
- What happens if my sample solvent is stronger than my mobile phase?
- Can I get a sharper peak by injecting my sample in a weaker injection solvent?
- How much sample can I inject on my LC column?
- What size threads are on the end fittings of my HPLC column?
- Why do most users privilege PEEK vs stainless steel fittings and tubing?
- What is the internal diameter of my LC tubing?
- Can I operate my column with a 100% aqueous mobile phase?
- How can the column performance be checked?
- How can the columns be cleaned or regenerated?
- What is the appropriate flow rate for the different column dimensions?
- What is the difference between dead volume and dwell volume?
- What is the shipping solvent for your HPLC columns?
- When should I use a gradient?
- What are the common buffers used in HPLC?
- Why is my chromatogram showing peak tailing, ghost peaks, fronting peaks, split peaks / shoulder peaks or rounded peaks?
- Why is it difficult to get reproducible retention times in normal phase chromatography?
- What is the difference between a monomeric and polymeric C18 bonded phase?
- How should the columns be stored?
- How to scale-up a column for preparative isolation/purification from analytical conditions?
- Should I run a gradient from 5-95% organic solvent or from 0-100%?
- How are column efficiency, peak asymmetry factor, tailing factor and resolution calculated?
- What to do when there are no peaks, small peaks or negative peaks?
- What to do when there is no flow?
- What is the cause of variable retention times, loss of resolution or selecticity change?
- What is the cause of baseline drift or noise?
-
>> Functionalized Silica
- What is the difference between silica and polymer?
- How should functionalized silica be handled?
- What are the storage conditions for the functionalized silica?
- Can functionalized silica be packed?
- Won’t the supported reagents leach since they are adsorbed and not bound?
- What is a supported reagent?
- Are all functionalized products endcapped?
- Does functionalized silica dissolve in methanol or any other standard organic solvents?
- Can different functionalized silicas be mixed?
- >> Solid Phase Extraction (SPE)
-
>> Thin Layer Chromatography
- How to switch to SiliCycle TLC Plates
- What type of TLC plate to choose?
- What type of TLC backing to choose?
- How to cut TLC plates on glass support?
- How to read a chromatogram?
- Stains for TLC Plates
- Streaking or elongated spot rather than a defined spot?
- Unable to see any spots on the TLC?
- How to monitor a reaction in presence of similar Rfs for both starting materials and product of interest?
- What is the co-spotting method?
- What to do if compounds stay too close to the baseline or solvent front?
- What is Solid Phase Extraction (SPE) ?
- Monomeric vs. polymeric C18
- What is Endcapping?
- What is Flow Chemistry?
- What is Microwave Chemistry?
- What is the "Catch and Release" technique?
- Why is the level of fines important?
- Are custom formats available?
- Silica Selection Guide
- >> Silica Analysis Descriptions
- Career
- Contact Us
© 2023 SiliCycle.
Follow us and get our news and promotions! 
Worldwide Headquarters SiliCycle Inc., 2500, Parc-Technologique Blvd, Quebec City (Quebec) G1P 4S6, CANADA
Phone: +1 418.874.0054 / Toll Free: +1 877.745.4292 (North America only)
Fax: +1 418.874.0355 E-mail: [email protected]
Copyright © 2023 SiliCycle Inc. All rights reserved.