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 APPLICATION NOTE 

Amine Sorbent for the Separation of Polar Compounds

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Due to the growing requirements for more specific and more efficient chromatographic sorbents, SiliCycle has developed a wide selection of chromatographic phases that can be prepared for bulk applications and packed in different sizes of Isco™, FlashMaster™ and Biotage™ compatible cartridges as well as SPE and 96 well plates formats.

Amine sorbent (Si-NH2) is a class of chromatographic functionality that has found its use in various types of applications such as the biological screening for drug of abuse and other basic compounds. Since Amine (NH2) is a weaker anion exchanger (WAX) than all of the commonly used tertiary amines (SAX), it can be used for purification of acidic compounds that would otherwise adsorb to the chromatographic sorbent during extraction. Alternatively, it has also been extensively used in reversed-phase chromatography for the separation of basic compounds that usually requires aminated mobile phase modifiers such as triethylamine (TEA) to prevent tailing or streaking of chromatographic peaks.

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Below is the list of our various formats based on SiliaBond Amine:

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  1. SiliaBond Amine (WAX) nec (R52130B)

    SiliaBond Amine (WAX) nec (R52130B)

    SiliaBond Amine (Si-NH2) is an effective scavenger of acid chlorides, sulfonyl chlorides, isocyanates and other electrophiles. Si-NH2 has been shown to be effective metal scavenger and catalyst for Knoevenagel reactions. SiliaBond Amine is also used in chromatography as normal phase sorbent.
  2. SiliaBond Amine (WAX) (R52030B)

    SiliaBond Amine (WAX) (R52030B)

    SiliaBond Amine (Si-NH2) is an effective scavenger of acid chlorides, sulfonyl chlorides, isocyanates and other electrophiles. Si-NH2 has been shown to be effective metal scavenger and catalyst for Knoevenagel reactions. SiliaBond Amine is also used in chromatography as normal phase sorbent.
  3. SiliaPrep SPE Cartridges Amine (WAX) (SPE-R52030B)

    SiliaPrep SPE Cartridges Amine (WAX) (SPE-R52030B)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.
  4. SiliaPrep SPE Cartridges Amine (WAX) nec (SPE-R52130B)

    SiliaPrep SPE Cartridges Amine (WAX) nec (SPE-R52130B)

    SiliaPrep Amine nec is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and norman phase conditions.
  5. SiliaPrep 96 Well Plates Amine (WAX), 40 - 63 µm, 60 Å (96W-R52030B)

    SiliaPrep 96 Well Plates Amine (WAX), 40 - 63 µm, 60 Å (96W-R52030B)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.
  6. SiliaPrep 96 Well Plates, Amine (WAX) nec, 40 - 63 µm, 60 Å (96W-R52130B)

    SiliaPrep 96 Well Plates, Amine (WAX) nec, 40 - 63 µm, 60 Å (96W-R52130B)

    When used at pH ≤ 7.8, Amine is protonated and may be used as a weak anion exchanger (WAX).
  7. SiliaSep OT Flash Cartridges (Open Top), Amine, 40 - 63 µm, 60 Å (FLH-R52030B-OT)

    SiliaSep OT Flash Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (FLH-R52030B-OT)

    SiliaSep OT flash cartridges, compatible with FlashMaster instrument. Good alternative for normal phase purification of compounds with basic properties. Useful for monosaccharides separation.
  8. SiliaPrepX Polymeric Well Plates, WAX, 85 µm, 60 Å (96W-P0020)

    SiliaPrepX Polymeric Well Plates, Amine (WAX), 85 µm, 60 Å (96W-P0020)

    SiliaPrepX WAX is a polystyrene-divinylbenzene copolymer functionalized by a weak anion exchanger used to catch and release strong acidic compounds (pKa <2). It is highly stable in organic solvents.
  9. SiliaPrepX WAX Polymeric SPE Cartridges, 85 µm, 60 Å (SPE-P0020)

    SiliaPrepX Polymeric SPE Cartridges, Amine (WAX), 85 µm, 60 Å (SPE-P0020)

    SiliaPrepX WAX is a polystyrene-divinylbenzene copolymer functionalized by a weak anion exchanger used to catch and release strong acidic compounds (pKa <2). It is highly stable in organic solvents.
  10. SiliaChrom Plus HPLC Columns, Amino, 3 µm, 100 Å (HPL-S52003E-A)

    SiliaChrom Plus HPLC Columns, Amine, 3 µm, 100 Å (HPL-S52003E-A)

    SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.
  11. SiliaChrom Plus HPLC Columns, Amine, 5 µm, 100 Å (HPL-S52005E-A)

    SiliaChrom Plus HPLC Columns, Amine (WAX), 5 µm, 100 Å (HPL-S52005E-A)

    SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.
  12. SiliaChrom Plus HPLC Columns, Amine, 10 µm, 100 Å (HPL-S52007E-A)

    SiliaChrom Plus HPLC Columns, Amine (WAX), 10 µm, 100 Å (HPL-S52007E-A)

    SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.
  13. SiliaChrom Plus HPLC Guard Cartridges, Amine, 3 µm, 100 Å (HPLG-S52003E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Amine (WAX), 3 µm, 100 Å (HPLG-S52003E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Amine, 3 µm.
  14. SiliaChrom Plus HPLC Guard Cartridges, Amine, 5 µm, 100 Å (HPLG-S52005E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Amine (WAX), 5 µm, 100 Å (HPLG-S52005E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Amine, 5 µm.
  15. SiliaChrom Plus HPLC Guard Cartridges, Amine, 10 µm, 100 Å (HPLG-S52007E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Amine (WAX), 10 µm, 100 Å (HPLG-S52007E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Amine, 10 µm.
  16. SiliaSep Flash Cartridges, Amine (WAX), 25 µm (FLH-52095D-A)

    SiliaSep PREMIUM Flash Cartridges, Amine (WAX), spherical 25 µm (FLH-52095D-A)

    The amine phase can be used either in normal phase for the purification of compounds with basic properties & for monosaccharides separation; or as a weak anion exchanger (pKa of 9.8). At pH 7.8 or below, the functional groups are positively charged. It facilitates the rapid release of very strong anions such as sulfonic acids that may be too strongly retained on SAX.

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SiliaSep PREMIUM - Flash Cartridges packed with Spherical Silica
E-PAK® - Fixed Bed Flow-through Purification Cartridges

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