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 APPLICATION NOTE 

Packing DAC Columns Using SiliaSphere Spherical Silica Gel

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Moving on from analytical columns method development to pilot or production scale has always represented a challenge. So too has the optimization of the packing procedures for large ID columns. Most columns for early method development are supplied prepacked by column manufacturers at relatively low costs.

In order to keep these costs minimal as well as for convenience and availability reasons, most process scale column users have elected to integrate Dynamic Axial Compression (DAC) column production in their own facilities whenever they need large ID columns (> 21.2 mm).

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Find below the list of SiliCycle's products involved in this Application Note:

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  1. SiliaSphere Spherical Silica Gel, 5 µm, 100 Å (S10005E-A)

    SiliaSphere Spherical Silica Gel, 5 µm, 100 Å (S10005E-A)

    Highly polar sorbent for the purification of non-polar and non-ionic compounds.
  2. SiliaSphere Spherical Silica Gel, 10 µm, 100 Å (S10007E-A)

    SiliaSphere Spherical Silica Gel, 10 µm, 100 Å (S10007E-A)

    Highly polar sorbent for the purification of non-polar and non-ionic compounds.
  3. SiliaSphere Spherical Silica Gel, C18 monomeric, 5 µm, 100 Å (S03205E-A)

    SiliaSphere Spherical Silica Gel, C18 monomeric, 5 µm, 100 Å (S03205E-A)

    Great start for method development. Presents the maximum retention of non-polar compounds. Usually used for peptides, pesticides, PCBs, PAHs, drugs, etc.
  4. SiliaSphere Spherical Silica Gel, C18 monomeric, 5 µm, 300 Å (S03205M)

    SiliaSphere Spherical Silica Gel, C18 monomeric, 5 µm, 300 Å (S03205M)

    Great start for method development. Presents the maximum retention of non-polar compounds. Usually used for peptides, pesticides, PCBs, PAHs, drugs, etc.
  5. SiliaSphere Spherical Silica Gel, C18 monomeric, 10 µm, 100 Å (S03207E-A)

    SiliaSphere Spherical Silica Gel, C18 monomeric, 10 µm, 100 Å (S03207E-A)

    Great start for method development. Presents the maximum retention of non-polar compounds. Usually used for peptides, pesticides, PCBs, PAHs, drugs, etc.
  6. SiliaSphere Spherical Silica Gel, C18 monomeric, 10 µm, 300 Å (S03207M)

    SiliaSphere Spherical Silica Gel, C18 monomeric, 10 µm, 300 Å (S03207M)

    Great start for method development. Presents the maximum retention of non-polar compounds. Usually used for peptides, pesticides, PCBs, PAHs, drugs, etc.
  7. SiliaSphere Spherical Silica Gel, C8 monomeric, 5 µm, 100 Å (S30805E-A)

    SiliaSphere Spherical Silica Gel, C8 monomeric, 5 µm, 100 Å (S30805E-A)

    Presents less retention compared to C18. Usually used for highly hydrophobic pesticides, small peptides and heavy drugs.
  8. SiliaSphere Spherical Silica Gel, C8 monomeric, 10 µm, 100 Å (S30807E-A)

    SiliaSphere Spherical Silica Gel, C8 monomeric, 10 µm, 100 Å (S30807E-A)

    Presents less retention compared to C18. Usually used for highly hydrophobic pesticides, small peptides and heavy drugs.
  9. SiliaSphere Spherical Silica Gel, C4 monomeric, 5 µm, 100 Å (S32705E-A)

    SiliaSphere Spherical Silica Gel, C4 monomeric, 5 µm, 100 Å (S32705E-A)

    Less retention than C18 and C8. Widely used for molecules, such as peptides, proteines and zwitterions (in 300 Å).
  10. SiliaSphere Spherical Silica Gel, C4 monomeric, 5 µm, 300 Å (S32705M)

    SiliaSphere Spherical Silica Gel, C4 monomeric, 5 µm, 300 Å (S32705M)

    Less retention than C18 and C8. Widely used for molecules, such as peptides, proteines and zwitterions (in 300 Å).
  11. SiliaSphere Spherical Silica Gel, C4 monomeric, 10 µm, 100 Å (S32707E-A)

    SiliaSphere Spherical Silica Gel, C4 monomeric, 10 µm, 100 Å (S32707E-A)

    Less retention than C18 and C8. Widely used for molecules, such as peptides, proteines and zwitterions (in 300 Å).
  12. SiliaSphere Spherical Silica Gel, C4 monomeric, 10 µm, 300 Å (S32707M)

    SiliaSphere Spherical Silica Gel, C4 monomeric, 10 µm, 300 Å (S32707M)

    Less retention than C18 and C8. Widely used for molecules, such as peptides, proteines and zwitterions (in 300 Å).
  13. SiliaSphere Spherical Silica Gel, Amine, 5 µm, 100 Å (S52005E-A)

    SiliaSphere Spherical Silica Gel, Amine (WAX) Polymeric, 5 µm, 100 Å (S52005E-A)

    Polar sorbent with basic character, less retention and a different selectivity for acidic/basic compounds.

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SiliaSep PREMIUM - Flash Cartridges packed with Spherical Silica
E-PAK® - Fixed Bed Flow-through Purification Cartridges

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