SiliCycle COVID-19 Statement

Follow SiliCycle on Facebook Follow SiliCycle on Twitter Follow SiliCycle on LinkedIn Watch our videos on Youtube
 APPLICATION NOTE 

Preparing Flash System and Cartridge for Reversed-Phase Chromatography

> back to Application Notes

What is the proper way to condition a reversed-phase flash column? The short answer is by performing a pre-wetting of the C18 bonded phase with 100 % methanol or 100 % acetonitrile.

For the full explanation and proper system preparation, read this Application Note.

Download our Application note

 

Find below the list of SiliCycle's products involved in this Application Note:

12 Item(s)

Set Descending Direction
per page

List  Grid 

  1. SiliaSep Flash Cartridges, C18 (17%) monomeric, 40 - 63 µm, 60 Å (FLH-R33230B)

    SiliaSep Flash Cartridges, C18 (17%), 40 - 63 µm, 60 Å (FLH-R33230B)

    Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
  2. SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
  3. SiliaSep PREMIUM Flash Cartridges, C18 Monomeric, 25 µm (FLH-03295D-A)

    SiliaSep PREMIUM Flash Cartridges, C18, spherical 25 µm (FLH-03295D-A)

    Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
  4. SiliaSep Flash Cartridges, C8 monomeric, 40 - 63 µm, 60 Å (FLH-R30830B)

    SiliaSep Flash Cartridges, C8, 40 - 63 µm, 60 Å (FLH-R30830B)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  5. SiliaSep Flash Cartridges, C4, 40 - 63 µm, 60 Å (FLH-R32030B)

    SiliaSep Flash Cartridges, C4, 40 - 63 µm, 60 Å (FLH-R32030B)

    Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
  6. SiliaSep Flash Cartridges, Phenyl, 40 - 63 µm, 60 Å (FLH-R34030B)

    SiliaSep Flash Cartridges, Phenyl, 40 - 63 µm, 60 Å (FLH-R34030B)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  7. SiliaSep Flash Cartridges, Pentafluorophenyl, 40 - 63 µm, 60 Å (FLH-R67530B)

    SiliaSep Flash Cartridges, Pentafluorophenyl (PFP), 40 - 63 µm, 60 Å (FLH-R67530B)

    For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
  8. SiliaSep Flash Cartridges, C8 Monomeric, 25 µm (FLH-30895D-A)

    SiliaSep PREMIUM Flash Cartridges, C8, spherical 25 µm (FLH-30895D-A)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  9. SiliaSep PREMIUM Flash Cartridges, C4, spherical 25 µm (FLH-32095D-A)

    SiliaSep PREMIUM Flash Cartridges, C4, spherical, 25 µm, 90 Å (FLH-32095D-A)

    Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
  10. SiliaSep PREMIUM Flash Cartridges, Phenyl, spherical, 25 µm, 90 Å (FLH-34095D-A)

    SiliaSep PREMIUM Flash Cartridges, Phenyl, spherical, 25 µm, 90 Å (FLH-34095D-A)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  11. SiliaSep PREMIUM Flash Cartridges, Pentafluorophenyl (PFP), 25 µm, 90 Å (FLH-67595D-A)

    SiliaSep PREMIUM Flash Cartridges, Pentafluorophenyl (PFP), 25 µm, 90 Å (FLH-67595D-A)

    For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
  12. SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.

12 Item(s)

Set Descending Direction
per page

List  Grid