Quick Overview of Column Chromatography

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Flash chromatography is a widely established chemical separation technique based on the polarity differences of compounds. To perform a successful purification, a method development is required to ensure the most efficient separation. This Application Note presents rules of thumb that can be followed in order to accomplish this goal.

Methodology

Prior to performing a separation with a chromatographic column, it is highly recommended to perform screening tests using thin layer chromatography (TLC) to determine the best conditions for the separation. From the results obtained with the TLC plates, one can then use the same conditions (solvent system and adsorbent) when moving to flash chromatography.

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