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Reversed-Phase Chromatography: General Introduction for Improved Method Development

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Today, more than 80 % of all analytical chromatographic separations are performed using reversed-phase sorbents as they have shown higher versatility in comparison to normal phase sorbents. Reversed-phase sorbents are used in a wide range of applications such as process purification, isolation of active biomolecules, analytical separation of drugs and metabolites as well as extraction of various contaminants in environmental samples.

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