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 APPLICATION NOTE 

Separation of Caffeine and Other Natural Stimulants Using Reversed-Phase C18 SiliaSep Flash Cartridges

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Xanthine derivatives are found in many food products because of their natural occurrence in tea leaves, cocoa beans, and coffee beans, to name a few. Caffeine, theobromine, and theophylline are methylated xanthines with similar chemical compositions and chemistries, making them a challenge to separate by chromatography.

This Application Note aims to separate these compounds by using reversed-phase C18 SiliaSep flash cartridges.

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Find below the list of SiliCycle's products involved in this Application Note:

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  1. SiliaSep Flash Cartridges, C18 (17%) monomeric, 40 - 63 µm, 60 Å (FLH-R33230B)

    SiliaSep Flash Cartridges, C18 (17%), 40 - 63 µm, 60 Å (FLH-R33230B)

    Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
  2. SiliaSep PREMIUM Flash Cartridges, C18 Monomeric, 25 µm (FLH-03295D-A)

    SiliaSep PREMIUM Flash Cartridges, C18, spherical 25 µm (FLH-03295D-A)

    Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.

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