Separation of Caffeine and Other Natural Stimulants Using Reversed-Phase C18 SiliaSep Flash Cartridges

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Xanthine derivatives are found in many food products because of their natural occurrence in tea leaves, cocoa beans, and coffee beans, to name a few. Caffeine, theobromine, and theophylline are methylated xanthines with similar chemical compositions and chemistries, making them a challenge to separate by chromatography.

This Application Note aims to separate these compounds by using reversed-phase C18 SiliaSep flash cartridges.

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Find below the list of SiliCycle's products involved in this Application Note: