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 APPLICATION NOTE 

Working with Amine (WAX) silica phase

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SiliaBond® Amine (Si-NH2) is a very versatile sorbent used for various applications. Known for its metal and electrophile scavenger properties as well its base reagent quality, it can also be used as a chromatographic phase and a Weak Anion Exchanger (WAX) sorbent which will be the focus of this application note. The Amine phase is available in bulk (SiliaBond®), in pre-packed flash cartridges (either irregular SiliaSep™ or spherical SiliaSep™ PREMIUM), in TLC plates (SiliaPlate), in HPLC columns (SiliaChrom® Plus) and in SPE cartridges (SiliaPrep) to help customers achieve their separation goals.

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  1. SiliaBond Amine (WAX) (R52030B)

    SiliaBond Amine (WAX) (R52030B)

    SiliaBond Amine (Si-NH2) is an effective scavenger of acid chlorides, sulfonyl chlorides, isocyanates and other electrophiles. Si-NH2 has been shown to be effective metal scavenger and catalyst for Knoevenagel reactions. SiliaBond Amine is also used in chromatography as normal phase sorbent.
  2. SiliaBond Amine (WAX) nec (R52130B)

    SiliaBond Amine (WAX) nec (R52130B)

    SiliaBond Amine (Si-NH2) is an effective scavenger of acid chlorides, sulfonyl chlorides, isocyanates and other electrophiles. Si-NH2 has been shown to be effective metal scavenger and catalyst for Knoevenagel reactions. SiliaBond Amine is also used in chromatography as normal phase sorbent.
  3. SiliaSep Flash Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (FLH-R52030B)

    SiliaSep Flash Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (FLH-R52030B)

    Good alternative for normal phase purification of compounds with basic properties, which would normally have to be purified by reversed-phase. Also a weak anion exchanger, with a pKa of 9.8. At pH 7.8 or below, the functional groups are positively charged. It facilitates the rapid release of very strong anions such as sulfonic acids that may be too strongly retained on SAX.
  4. SiliaSep OT Flash Cartridges (Open Top), Amine, 40 - 63 µm, 60 Å (FLH-R52030B-OT)

    SiliaSep OT Flash Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (FLH-R52030B-OT)

    SiliaSep OT flash cartridges, compatible with FlashMaster instrument. Good alternative for normal phase purification of compounds with basic properties. Useful for monosaccharides separation.
  5. SiliaSep Pre-Packed Solid-Load Cartridges, Amine (WAX) (SPL-R52030B)

    SiliaSep Pre-Packed Solid-Load Cartridges, Amine (WAX) (SPL-R52030B)

    Designed to be used with SiliaSep flash cartridges for sample loading by liquid injection
  6. SiliaSep Flash Cartridges, Amine (WAX), 25 µm (FLH-52095D-A)

    SiliaSep PREMIUM Flash Cartridges, Amine (WAX), spherical 25 µm (FLH-52095D-A)

    The amine phase can be used either in normal phase for the purification of compounds with basic properties & for monosaccharides separation; or as a weak anion exchanger (pKa of 9.8). At pH 7.8 or below, the functional groups are positively charged. It facilitates the rapid release of very strong anions such as sulfonic acids that may be too strongly retained on SAX.

  7. SiliaPrep SPE Cartridges Amine (WAX) (SPE-R52030B)

    SiliaPrep SPE Cartridges Amine (WAX) (SPE-R52030B)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.
  8. SiliaPrep 96 Well Plates Amine (WAX), 40 - 63 µm, 60 Å (96W-R52030B)

    SiliaPrep 96 Well Plates Amine (WAX), 40 - 63 µm, 60 Å (96W-R52030B)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.
  9. Mini-SiliaPrep SPE Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (SPS-R52030B)

    Mini-SiliaPrep SPE Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (SPS-R52030B)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.
  10. SiliaPrep LRV SPE Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (SPC-R52030B)

    SiliaPrep LRV SPE Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (SPC-R52030B)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and in normal phase conditions.
  11. SiliaChrom Plus HPLC Columns, Amino, 3 µm, 100 Å (HPL-S52003E-A)

    SiliaChrom Plus HPLC Columns, Amine, 3 µm, 100 Å (HPL-S52003E-A)

    SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.
  12. SiliaChrom Plus HPLC Columns, Amine, 5 µm, 100 Å (HPL-S52005E-A)

    SiliaChrom Plus HPLC Columns, Amine (WAX), 5 µm, 100 Å (HPL-S52005E-A)

    SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.
  13. SiliaChrom Plus HPLC Columns, Amine, 10 µm, 100 Å (HPL-S52007E-A)

    SiliaChrom Plus HPLC Columns, Amine (WAX), 10 µm, 100 Å (HPL-S52007E-A)

    SiliaChrom Plus Amine columns are very popular for sugars analysis (carbohydrates, glycosides and oligosaccharides). They represent a good alternative for normal phase separation of compounds with acidic/basic properties. SiliaChrom Plus Amine columns can also be used in Ion Exchange Mode, for retention & release of very strong anions such as sulfonic acids.
  14. SiliaChrom Plus HPLC Guard Cartridges, Amine, 3 µm, 100 Å (HPLG-S52003E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Amine (WAX), 3 µm, 100 Å (HPLG-S52003E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Amine, 3 µm.
  15. SiliaChrom Plus HPLC Guard Cartridges, Amine, 5 µm, 100 Å (HPLG-S52005E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Amine (WAX), 5 µm, 100 Å (HPLG-S52005E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Amine, 5 µm.
  16. SiliaChrom Plus HPLC Guard Cartridges, Amine, 10 µm, 100 Å (HPLG-S52007E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Amine (WAX), 10 µm, 100 Å (HPLG-S52007E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Amine, 10 µm.
  17. SiliaPlate HPTLC Plates, Glass-Backed, Amine (NH2), 150 µm, 10 x 10 cm, F254 (TLG-R52011B-203)
  18. SiliaPlate HPTLC Plates, Glass-Backed, Amine (NH2), 150 µm, 20 x 20 cm, F254 (TLG-R52011B-303)
  19. SiliaPlate HPTLC Plates, Glass-Backed, Amine (NH2), Optimized for KMnO4, 200 µm, 10 x 10 cm, F254 (TLG-R52014BK-213)
  20. SiliaPlate HPTLC Plates, Glass-Backed, Amine (NH2), Optimized for KMnO4, 200 µm, 20 x 20 cm, F254 (TLG-R52014BK-313)
  21. SiliaPlate TLC Plates, Aluminum-Backed, Amine (NH2), Optimized for KMnO4, 150 µm, 4 x 8 cm, F254 (TLA-R52014BK-005)
  22. SiliaPlate HPTLC Plates, Glass-Backed	Amine (NH2), 150 µm, 10 x 20 cm, F254 (TLG-R52011B-723)
  23. SiliaPlate HPTLC Plates, Glass-Backed, Amine (NH2), Optimized for KMnO4, 200 µm, 10 x 20 cm, F254 (TLG-R52014BK-713)

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