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 APPLICATION NOTE 

Working with Cyano silica phase

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SiliaBond® Cyano (Si-CN) is a very versatile sorbent due to its polarity marking the separation between the polar and non-polar phases. This sorbent is particularly useful to reduce retention times and improve peak shapes when dealing with extremes in the polarity scale for both normal and reversed-phase chromatography. In fact, it is the least retentive polar sorbent in normal phase chromatography and the least retentive of the non‑polar sorbents in reversed‑phase chromatography. The Cyano phase is available in bulk (SiliaBond®), in pre-packed flash cartridges (either irregular SiliaSep™ or spherical SiliaSep™ PREMIUM), in TLC plates (SiliaPlate™), in HPLC columns (SiliaChrom® Plus Cyano) and in SPE cartridges (SiliaPrep™) to help customers achieve their separation goals.

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  1. SiliaBond Cyano (CN, 7%C) (R38030B)

    SiliaBond Cyano (CN, 7%C) (R38030B)

    SiliaBond Cyano (Si-CN) can be used both in normal and reversed-phase chromatography as its polarity marks the separation between the polar and non-polar phases. SiliaBond Cyano is the least retentive polar sorbent in normal phase chromatography and the least retentive of the non-polar sorbents in reversed-phase chromatography.
  2. SiliaBond Cyano (CN, 7%C) nec (R38130B)

    SiliaBond Cyano (CN, 7%C) nec (R38130B)

    SiliaBond Cyano (Si-CN) can be used both in normal and reversed-phase chromatography as its polarity marks the separation between the polar and non-polar phases. SiliaBond Cyano is the least retentive polar sorbent in normal phase chromatography and the least retentive of the non-polar sorbents in reversed-phase chromatography.
  3. SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
  4. SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
  5. SiliaSep Pre-Packed Solid-Load Cartridges, Cyano (SPL-R38030B)

    SiliaSep Pre-Packed Solid-Load Cartridges, Cyano (SPL-R38030B)

    Designed to be used with SiliaSep flash cartridges for sample loading by liquid injection
  6. SiliaPrep SPE Cartridges Cyano (CN) (SPE-R38030B)

    SiliaPrep SPE Cartridges Cyano (CN) (SPE-R38030B)

    SiliaPrep Cyano is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to extract acidic, neutral, basic compounds from aqueous solutions.
  7. SiliaPrep SPE Cartridges Cyano nec (SPE-R38130B)

    SiliaPrep SPE Cartridges Cyano nec (SPE-R38130B)

    SiliaPrep Cyano nec is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to ectract acidic, neutral, basic compounds from aqueous solutions.
  8. SiliaPrep 96 Well Plates, Cyano (CN), 40 - 63 µm, 60 Å (96W-R38030B)

    SiliaPrep 96 Well Plates, Cyano (CN), 40 - 63 µm, 60 Å (96W-R38030B)

    SiliaPrep Cyano is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to extract acidic, neutral, basic compounds from aqueous solutions.
  9. SiliaPrep 96 Well Plates, Cyano nec, 40 - 63 µm, 60 Å (96W-R38130B)

    SiliaPrep 96 Well Plates, Cyano nec, 40 - 63 µm, 60 Å (96W-R38130B)

    SiliaPrep Cyano nec is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to ectract acidic, neutral, basic compounds from aqueous solutions.
  10. SiliaSep OT Flash Cartridges (Open Top), Cyano, 40 - 63 µm, 60 Å (FLH-R38030B-OT)

    SiliaSep OT Flash Cartridges (Open Top), Cyano, 40 - 63 µm, 60 Å (FLH-R38030B-OT)

    SiliaSep OT flash cartridges, compatible with FlashMaster instrument. Versatile sorbent that can be used either as normal or reversed-phase. Less polar sorbent compared to silica. Used for the purification of polar organic compounds.
  11. SiliaPrep Tips Micro-SPE Cartridges Cyano (CN) (SPET-CN-T)

    SiliaPrep Tips Micro-SPE Cartridges Cyano (CN) (SPET-CN-T)

    Both polar and hydrophobic character. Mainly used for hydrophilic and neutral molecules enrichment.
  12. SiliaPrep LRV SPE Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (SPC-R38030B)

    SiliaPrep LRV SPE Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (SPC-R38030B)

    SiliaPrep Cyano is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to extract acidic, neutral, basic compounds from aqueous solutions.
  13. Mini-SiliaPrep SPE Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (SPS-R38030B)

    Mini-SiliaPrep SPE Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (SPS-R38030B)

    SiliaPrep Cyano is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to extract acidic, neutral, basic compounds from aqueous solutions.
  14. SiliaChrom Plus HPLC Columns, Cyano, 3 µm, 100 Å (HPL-S38003E-A)

    SiliaChrom Plus HPLC Columns, Cyano, 3 µm, 100 Å (HPL-S38003E-A)

    SiliaChrom Plus Cyano columns can be used both in normal and reversed-phase. Less polar than silica, they will help retain organic compounds with intermediate to extreme polarity. Whether you need to analyse carbohydrates, glycosides, cyclosporine or any other carboxyl / carbonyl / amine containing molecules, SiliaChrom Plus Cyano is a good choice.
  15. SiliaChrom Plus HPLC Columns, Cyano, 5 µm, 100 Å (HPL-S38005E-A)

    SiliaChrom Plus HPLC Columns, Cyano, 5 µm, 100 Å (HPL-S38005E-A)

    SiliaChrom Plus Cyano columns can be used both in normal and reversed-phase. Less polar than silica, they will help retain organic compounds with intermediate to extreme polarity. Whether you need to analyse carbohydrates, glycosides, cyclosporine or any other carboxyl / carbonyl / amine containing molecules, SiliaChrom Plus Cyano is a good choice.
  16. SiliaChrom Plus HPLC Columns, Cyano, 10 µm, 100 Å (HPL-S38007E-A)

    SiliaChrom Plus HPLC Columns, Cyano, 10 µm, 100 Å (HPL-S38007E-A)

    SiliaChrom Plus Cyano columns can be used both in normal and reversed-phase. Less polar than silica, they will help retain organic compounds with intermediate to extreme polarity. Whether you need to analyse carbohydrates, glycosides, cyclosporine or any other carboxyl / carbonyl / amine containing molecules, SiliaChrom Plus Cyano is a good choice.
  17. SiliaChrom Plus HPLC Guard Cartridges, Cyano, 3 µm, 100 Å (HPLG-S38003E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Cyano, 3 µm, 100 Å (HPLG-S38003E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Cyano, 3 µm.
  18. SiliaChrom Plus HPLC Guard Cartridges, Cyano, 10 µm, 100 Å (HPLG-S38005E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Cyano, 5 µm, 100 Å (HPLG-S38005E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Cyano, 5 µm.
  19. SiliaChrom Plus HPLC Guard Cartridges, Cyano, 10 µm, 100 Å (HPLG-S38007E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Cyano, 10 µm, 100 Å (HPLG-S38007E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Cyano, 10 µm.
  20. SiliaSphere Spherical Silica Gel, Cyano (CN), 5 µm, 120 Å (S38005G-A)

    SiliaSphere Spherical Silica Gel, Cyano (CN), 5 µm, 120 Å (S38005G-A)

    Normal: less polar sorbent used for the purification of polar organic compounds. Reversed: Moderate non-polar sorbent with less hydrophobicity. Purification of cyclosporine and carbohydrates.

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