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 APPLICATION NOTE 

Working with Diol silica phase

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SiliaBond® Diol nec (Si-Diol) is a chromatographic phase mainly used as a polar sorbent in normal phase and aqueous size exclusion chromatography. For example, Si-Diol can be used for difficult separations of low to medium polarity samples. Furthermore, its effectiveness is well known for mono and polysaccharide separations.
As a bonus, it is the phase of choice to use in HILIC mode. Lastly, it can act as a boronic acid scavenger (in the same way SiliaMetS® DEAM (R54430B) does). Available in bulk (SiliaBond®), in pre-packed flash cartridges (either irregular SiliaSep™ or spherical SiliaSep™ PREMIUM), in TLC plates (SiliaPlate™), in HPLC columns (SiliaChrom® Plus Diol) and in SPE cartridges (SiliaPrep™) to help customers achieve their separation goals.

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  1. SiliaBond Diol nec (R35030B)

    SiliaBond Diol nec (R35030B)

    SiliaBond Diol may also be used as polar sorbent in normal phase and aqueous size exclustion chromatography. Like bare silica, SiliaBond Diol has the ability to form hydrogen bonds and the capacity to separate structural isomers. Since most of its surface is covered with organic functions, the SiliaBond Diol absorbs less water, which leads to a more reproducible activity. It is also the sorbent of choice when working in normal phase in the presence of water.
  2. SiliaSep Flash Cartridges, Diol, 25 µm (FLH-35095D-A)

    SiliaSep PREMIUM Flash Cartridges, Diol, spherical 25 µm (FLH-35095D-A)

    Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.
  3. SiliaSep Flash Cartridges, Diol nec, 40 - 63 µm, 60 Å (FLH-R35030B)

    SiliaSep Flash Cartridges, Diol nec, 40 - 63 µm, 60 Å (FLH-R35030B)

    Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.
  4. SiliaSep OT Flash Cartridges (Open Top), Diol nec, 40 - 63 µm, 60 Å (FLH-R35030B-OT)

    SiliaSep OT Flash Cartridges (Open Top), Diol nec, 40 - 63 µm, 60 Å (FLH-R35030B-OT)

    SiliaSep OT flash cartridges, compatible with any FlashMaster instrument. Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.
  5. SiliaSep Pre-Packed Solid-Load Cartridges, Diol (SPL-R35030B)

    SiliaSep Pre-Packed Solid-Load Cartridges, Diol (SPL-R35030B)

    Designed to be used with SiliaSep flash cartridges for sample loading by liquid injection
  6. SiliaPrep SPE Cartridges Diol (SPE-R35030B)

    SiliaPrep SPE Cartridges Diol nec (SPE-R35030B)

    SiliaPrep Diol nec is a moderate polar sorbent presenting neutral character used to extract polar compounds from non-polar solvents, structural isomers. Alternative to silica when the acidic character is problematic.
  7. SiliaPrep 96 Well Plates, Diol nec, 40 - 63 µm, 60 Å (96W-R35030B)

    SiliaPrep 96 Well Plates, Diol nec, 40 - 63 µm, 60 Å (96W-R35030B)

    SiliaPrep Diol nec is a moderate polar sorbent presenting neutral character used to extract polar compounds from non-polar solvents, structural isomers. Alternative to silica when the acidic character is problematic.
  8. SiliaPrep LRV SPE Cartridges, Diol nec, 40 - 63 µm, 60 Å (SPC-R35030B)

    SiliaPrep LRV SPE Cartridges, Diol nec, 40 - 63 µm, 60 Å (SPC-R35030B)

    SiliaPrep Diol nec is a moderate polar sorbent presenting neutral character used to extract polar compounds from non-polar solvents, structural isomers. Alternative to silica when the acidic character is problematic.
  9. Mini-SiliaPrep SPE Cartridges, Diol nec, 40 - 63 µm, 60 Å (SPS-R35030B)

    Mini-SiliaPrep SPE Cartridges, Diol nec, 40 - 63 µm, 60 Å (SPS-R35030B)

    SiliaPrep Diol nec is a moderate polar sorbent presenting neutral character used to extract polar compounds from non-polar solvents, structural isomers. Alternative to silica when the acidic character is problematic.
  10. SiliaPlate HPTLC Plates, Glass-Backed, Diol, Optimized for KMnO4, 200 µm, 10 x 10 cm, F254 (TLG-R35014BK-213)
  11. SiliaPlate HPTLC Plates, Glass-Backed, Diol, Optimized for KMnO4, 200 µm, 20 x 20 cm, F254 (TLG-R35014BK-313)
  12. SiliaPlate HPTLC Plates, Glass-Backed, Diol, Optimized for KMnO4, 200 µm, 10 x 20 cm, F254 (TLG-R35014BK-713)
  13. SiliaPlate HPTLC Plates, Glass-Backed, Diol, 200 µm, 10 x 20 cm, F254 (TLG-R35011B-713)
  14. SiliaChrom Plus HPLC Columns, Diol, 3 µm, 100 Å (HPL-S35003E-A)

    SiliaChrom Plus HPLC Columns, Diol, 3 µm, 100 Å (HPL-S35003E-A)

    SiliaChrom Plus Diol columns are ideal for difficult separations of low to medium polarity samples. They can be used for sugars analysis (carbohydrates, glycosides and oligosaccharides) and vitamins analysis. SiliaChrom Plus Diol columns are also compatible with HILIC mode (Hydrophilic Interaction LIquid Chromatography).
  15. SiliaChrom Plus HPLC Columns, Diol, 5 µm, 100 Å (HPL-S35005E-A)

    SiliaChrom Plus HPLC Columns, Diol, 5 µm, 100 Å (HPL-S35005E-A)

    SiliaChrom Plus Diol columns are ideal for difficult separations of low to medium polarity samples. They can be used for sugars analysis (carbohydrates, glycosides and oligosaccharides) and vitamins analysis. SiliaChrom Plus Diol columns are also compatible with HILIC mode (Hydrophilic Interaction LIquid Chromatography).
  16. SiliaChrom Plus HPLC Columns, Diol, 10 µm, 100 Å (HPL-S35007E-A)

    SiliaChrom Plus HPLC Columns, Diol, 10 µm, 100 Å (HPL-S35007E-A)

    SiliaChrom Plus Diol columns are ideal for difficult separations of low to medium polarity samples. They can be used for sugars analysis (carbohydrates, glycosides and oligosaccharides) and vitamins analysis. SiliaChrom Plus Diol columns are also compatible with HILIC mode (Hydrophilic Interaction LIquid Chromatography).
  17. SiliaChrom Plus HPLC Guard Cartridges, Diol, 3 µm, 100 Å (HPLG-S35003E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Diol, 3 µm, 100 Å (HPLG-S35003E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Diol, 3 µm.
  18. SiliaChrom Plus HPLC Guard Cartridges, Diol, 5 µm, 100 Å (HPLG-S35005E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Diol, 5 µm, 100 Å (HPLG-S35005E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Diol, 5 µm.
  19. SiliaChrom Plus HPLC Guard Cartridges, Diol, 10 µm, 100 Å (HPLG-S35007E-A)

    SiliaChrom Plus HPLC Guard Cartridges, Diol, 10 µm, 100 Å (HPLG-S35007E-A)

    Adequate Guard Cartridges to use with our SiliaChrom Plus HPLC Columns, Diol, 10 µm.
  20. SiliaSphere Spherical Silica Gel, Diol, 5 µm, 60 Å (S35005B)
  21. SiliaSphere Spherical Silica Gel, Diol, 5 µm, 100 Å (S35005E-A)
  22. SiliaSphere Spherical Silica Gel, Diol, 10 µm, 60 Å (S35007B)
  23. SiliaSphere Spherical Silica Gel, Diol, 10 µm, 100 Å (S35007E-A)
  24. SiliaSphere Spherical Silica Gel, Diol, 10 µm, 1000 Å (S35007T)
  25. SiliaSphere Spherical Silica Gels, Diol, 20 µm, 100 Å (S35009E-A)

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