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Quick Overview Of Column Chromatography

Before performing a separation on a chromatographic column, it is highly recommended to make different tests on thin layer chromatography (TLC) to find the best conditions for the separation. It is then important to use the same solvent system and adsorbent afterwards, on flash chromatography.

This document includes the principal common steps for performing flash chromatography:

2-Theory of separation
3-Choice of solvent
4-Loading the column
5-Solvent equilibration
6-Loading of the sample
7-Column capacity

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