Stains For Thin Layer Chromatography (TLC) Plates
This document includes common recipes of different stains used for developing thin layer chromatography (TLC).
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FAQ
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>> Metal & Organic Scavengers
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- How to purify your API from organic contaminants?
- What is a supported scavenger?
- What quantity of scavenger should be used?
- At what temperature should the scavenger be used?
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- Reaction time vs scavenger quantity
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>> HPLC
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- Can I get a sharper peak by injecting my sample in a weaker injection solvent?
- How much sample can I inject on my LC column?
- What size threads are on the end fittings of my HPLC column?
- Why do most users privilege PEEK vs stainless steel fittings and tubing?
- What is the internal diameter of my LC tubing?
- Can I operate my column with a 100% aqueous mobile phase?
- How can the column performance be checked?
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- What is the appropriate flow rate for the different column dimensions?
- What is the difference between dead volume and dwell volume?
- What is the shipping solvent for your HPLC columns?
- When should I use a gradient?
- What are the common buffers used in HPLC?
- Why is my chromatogram showing peak tailing, ghost peaks, fronting peaks, split peaks / shoulder peaks or rounded peaks?
- Why is it difficult to get reproducible retention times in normal phase chromatography?
- What is the difference between a monomeric and polymeric C18 bonded phase?
- How should the columns be stored?
- How to scale-up a column for preparative isolation/purification from analytical conditions?
- Should I run a gradient from 5-95% organic solvent or from 0-100%?
- How are column efficiency, peak asymmetry factor, tailing factor and resolution calculated?
- What to do when there are no peaks, small peaks or negative peaks?
- What to do when there is no flow?
- What is the cause of variable retention times, loss of resolution or selecticity change?
- What is the cause of baseline drift or noise?
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>> Functionalized Silica
- What is the difference between silica and polymer?
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- >> Solid Phase Extraction (SPE)
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>> Thin Layer Chromatography
- How to switch to SiliCycle TLC Plates
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- Stains for TLC Plates
- Streaking or elongated spot rather than a defined spot?
- Unable to see any spots on the TLC?
- How to monitor a reaction in presence of similar Rfs for both starting materials and product of interest?
- What is the co-spotting method?
- What to do if compounds stay too close to the baseline or solvent front?
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