How to run a TLC separation?

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The most commonly used method to perform thin layer chromatography separation is to place vertically the TLC plate inside a sealed developing chamber to ensure solvent saturation. Place approximately 0.5 cm of the suitable solvent system inside the chamber. Slowly place the TLC inside the chamber and allow the eluent to travel up the plate until it gets to 1 cm from the top of the plate. Immediately remove the plate and draw a line along the solvent front.

Note: for optimal solvent saturation, a filter paper can be added inside the TLC chamber. This also prevents eluent evaporation. The solvent level needs to be below the baseline; otherwise the spots will be dissolved.